An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how large will the object appear (what are the magnified dimensions of the object)?
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- Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.3. Which term refers to the clarity of the image? (a) magnification, (b) resolution, (c) contrast
- Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?4. Study the microscope drawing on page 11 and the description of the parts on the opposite page and then answer these questions. [a]What is the total magnification when the 4ox objective lens is being used? [b]Which objective lens "sees" the largest area of the slide? [c]Which focusing knob is used at high power? [d]What devise is used to change the amount of light coming through the object? devices are aná1. In the following table, fill in the missing information in order to calculate the Total Magnifica- tion for each objective lens. Use the information provided in the laboratory exercise to help you. Magnification of Magnification of Objective Lens Total Magnification Objective Lens Ocular Lens Lowest Power 4X Higher Power 10X Greater Power 40X Highest Power 100X 2. What would the total magnification be if a microscope had 15X ocular lenses and 4X, 10x, 45X and 95X objective lenses? Insert the correct information in the following table. Magnification of Objective Lens Magnification of Objective Lens Total Magnification Ocular Lens 4X Objective lens 10X Objective lens 45X Objective lens 95X Objective Lens
- Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length um1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
- The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm