Analyze the following genotypes of the Lac Operon in E. coli. In each case, determine whether Beta-galactosidase (enzyme that digests lactose) and permease (membrane channel that brings lactose into the cell) would be made in the presence or absence of lactose. For the purpose of this question, ignore glucose levels. Simply select 'YES' if the proteins are made or 'NO' if they are not . (Refer to the Lac Operon diagram for the meaning of the letters in the genotypes. Remember a "plus" superscript indicates normal functioning part of operon or repressor gene, a "minus" indicates loss of function, "OC" means "operator- constitutive", and "IS" means "super-repressor") Absence of Lactose Presence of Lactose Genotype В-gal Permease В -gal Permease IS pt 0 z+ y-/ I† P+ 0C z¯ y+ It p+ 0C z+ y+/ I* P¯ 0 Z Y It P 0C Z Y+/ I¯ P* 0 z+ y I+ pt 0 z Y+/ I¯ p+ 0 z+ Y

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Analyze the following genotypes of the Lac Operon in E. coli. In each case, determine
whether Beta-galactosidase (enzyme that digests lactose) and permease
(membrane channel that brings lactose into the cell) would be made in the presence
or absence of lactose. For the purpose of this question, ignore glucose levels. Simply
select 'YES' if the proteins are made or 'NO' if they are not .
(Refer to the Lac Operon diagram for the meaning of the letters in the genotypes.
Remember a "plus" superscript indicates normal functioning part of operon or
repressor gene, a "minus" indicates loss of function, "OC" means "operator-
constitutive", and "IS" means "super-repressor")
Absence of Lactose Presence of Lactose
Genotype
В-gal
Permease
В -gal
Permease
IS pt 0
z+ Y-/ I† p+ 0C z¯ y+
It p+ 0C z+ y+/ I* P¯ 0 Z- Y
It P 0C Z- Y+/ I¯ p* 0 z+ y
It pt o z Y+/ I¯ p+ 0 z+ Y
Transcribed Image Text:Analyze the following genotypes of the Lac Operon in E. coli. In each case, determine whether Beta-galactosidase (enzyme that digests lactose) and permease (membrane channel that brings lactose into the cell) would be made in the presence or absence of lactose. For the purpose of this question, ignore glucose levels. Simply select 'YES' if the proteins are made or 'NO' if they are not . (Refer to the Lac Operon diagram for the meaning of the letters in the genotypes. Remember a "plus" superscript indicates normal functioning part of operon or repressor gene, a "minus" indicates loss of function, "OC" means "operator- constitutive", and "IS" means "super-repressor") Absence of Lactose Presence of Lactose Genotype В-gal Permease В -gal Permease IS pt 0 z+ Y-/ I† p+ 0C z¯ y+ It p+ 0C z+ y+/ I* P¯ 0 Z- Y It P 0C Z- Y+/ I¯ p* 0 z+ y It pt o z Y+/ I¯ p+ 0 z+ Y
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