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Q. based on the graph, the absorbance of caffeine using UV-vis spectroscopy is at ________.
CHOICES:
A. |
320 nm |
B. |
260 nm |
C. |
260-290 nm |
D. |
275 nm |
E. |
260-300 nm |
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- 4. Determine the percent transmittance of ß-carotene, C40H56 that has an absorbance of 0.5 when analyse using a UV-Visible spectrophotometer. A. 6.83% B. 31.6% C. 0.32% D. 68.3% 5. The equation of Beer Lambert's Law is A = abc. If the 'b' is distance, 'a' is absorptivity, and 'A' is absorbance, what does the 'c' represent? 7. A. Intensity B. Analyte concentration C. Analyte transmittance D. Frequency 6. All the following is an example of photon detectors EXCEPT: A. Vacuum phototube B. Silicon photodiodes C. Bolometers D. Photomultiplier tubes C=0 bond stretch occurs at 1750 cm¹ and C-O stretch occurs at 1100 cm¹ because: A. The C-O has a single bond which is a stronger bond, so it vibrates at a higher frequency than the C=O. B. The C-O has a single bond which is a stronger bond, so it vibrates at a lower frequency than the C=O. C. The C=0 has a double bond which is a stronger bond, so it vibrates at a lower frequency than the C-O. D. The C-O has a double bond which is a stronger bond,…A calibration curve is made for the absorbance as a function of concentration of blue dye (in mg/L). The slope of the curve is 0.131; the y-intercept is -0.01869; the correlation is 0.993. If a consumer sample has an absorbance of 0.120 on the same instrument, what is its concentration of blue dye, in mg/L? a. 0.034 b. 0.935 c. 0.993 d. 1.065. Determine the percent transmittance of B-carotene, C40H56 that has an absorbance of 0.5 when analyse using a UV-Visible spectrophotometer. A. 6.83% B. 31.6% C. 0.32 % D. 68.3% 6. The equation of Beer Lambert's Law is A = abc. If the 'b' is distance, 'a' is absorptivity, and 'A' is absorbance, what does the 'c' represent? A. Intensity B. Analyte concentration C. Analyte transmittance D. Frequency 7. All the following is an example of photon detectors EXCEPT: A. Vacuum phototube B. Silicon photodiodes C. Bolometers D. Photomultiplier tubes 8. C=O bond stretch occurs at 1750 cm1 and C-O stretch occurs at 1100 cm1 because: A. The C-O has a single bond which is a stronger bond, so it vibrates at a higher frequency than the C=O. B. The C-O has a single bond which is a stronger bond, so it vibrates at a lower frequency than the C=O. C. The C=O has a double bond which is a stronger bond, so it vibrates at a lower frequency than the C-O. D. The C=O has a double bond which is a stronger bond,…
- Express the absorbance of 0.66 in terms of percent transmittance. A. 66% В. 22% С. 78% D. 34% E. 33%A. dependent variable independent variable B) Graph B C) Graph C D) Graph D E) Graph E D. In 700 wavelength (nm) 400 B. of 700 400 wavelength E. Which graph above best illustrates the relationship between the concentration of a substance and the height of its absorbance peak? A) Graph A C. time timeWhich of the following is not a step in Atomic Absorption Spectroscopy (AAS)? A. Atoms absorb light. B. A solution is vaporized C. A calibration curve is constructed. D. Particles are adsorbed onto a stationary phase.
- Food dye spectroscopy 2. At what wavelengths should the readings be taken? What instrumentation will you utilize with a solution of a known dye to determine the wavelength for your analysis?True or False 1. Photodiode arrays are devices within the instrument that help amplify the signal of a sample. 2. Atomic spectroscopy requires the atomization of a sample, which involves nebulization and eventually volatilization to form an elemental vapor. 3. Organic compounds are normally analyzed in atomic spectroscopy despite their small electronic transition range.Solutions that contain the FeSCN2+ ion are blood red (you will see this at the end of the second quarter of lab). Consider the regions of the visible spectrum in which the compound should absorb light and prepare a rough sketch of absorbance vs. wavelength for each solution. Label λ max on the graph. (Is this question asking for the graph of my data at the end)
- Describe the basic design difference between a spectrometer for absorption measurements and one for emission studies?1. What is the concentration of the dye in a solution with a percent transmittance reading of 61.6% at 570 nm? 1a. If the solution in Part 1 was made by taking 10.00 mL of a stock solution and diluting it to 100.00 mL, what is the concentration of the dye in the stock solution?2. Calculate the absorbance value (A) for a solution that absorbs 89.5% of the light striking it. (Hint: What is the percent transmittance? Review the background reading)