Calculate the amount of phycocyanin in Sample x in mg where A620=0.191 and A650=0.093, taking into account the dilution factor which is 100 and the total volume of extract which is 50 Note your answer to 2 decimal places.
Q: In the work-up, the reaction mixture is neutralized with 3M sulfuric acid to a final pH of 1-2. Why…
A: PH of a substance or reaction is defined as the negative logarithm of the hydrogen ion…
Q: If a sample of beeswax is found to have an acid number of 15.5 and saponification value of 71.2, the…
A: Given values: Saponification value = 71.2 Acid number = 15.5
Q: Quinoline is isolated from a reaction mixture by the process of steam distillation. A mixture of…
A: The numerical is based on Raoult's Law Relative lowering of vapour pressure = mole fraction of…
Q: Prepare 200 ml of a 0.125 M stock of NaCl from a 1 M concentrated stock solution of NaCl.
A: An artificial nutrient medium contains nutritional components like sugars, salts, amino acids, etc.…
Q: The nitrite in a series of standard solutions (mg/L n = 5) are converted to azo dye and the slope of…
A: A standard curve is made to ensure the correct determination of the unknown concentration of the…
Q: MD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of…
A: Morphine has analgesic properties and is used to relieve pain. The drug belongs to a class of drugs…
Q: Provide a chemical test/reagent that would differentiate each of the following pairs of compounds.…
A: Asked : Reagent and indication of positive test
Q: Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest…
A: Chromatography is a technique for separating different components of a sample, where the analyte is…
Q: A chromatographic separation of four compounds gave the following results. The dead time (tm) is…
A: Chromatography is a technique for separating a mixture in the laboratory. The combination is…
Q: What is the name of the sugar which produces yellow crystals after osazone test and have…
A:
Q: How will you prepare a 5% BSA solution? Show all your steps.
A: BSA stands for bovine serum albumin, which is a protein extracted from cows' serum albumin. In lab…
Q: You must make a choice between two chromatographic systems for the analysis of a specific compound.…
A: Partition coefficient can be determined as the ratio of the two-phase that is the stationary and…
Q: Using 25 µL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ul
A: Firstly, we convert the 25ul into ml . i.e. 25 ul = 0.03 ml Then, there will be the multiplication…
Q: Prepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the…
A: Final concentration can be calculated from the formula C1V1=C2V2 Where C1= initial concentration V1=…
Q: Discuss why sodium hydroxide may be used in conjunction with triethanolamine to react with stearic…
A: Vanishing cream from the name indicates that the cream gets vanished when applied onto the skin. The…
Q: Calculate the volume of BSA stock that will be required to make the standard solutions needed to…
A: A stock solution is a concentrated solution that is diluted to make a working solution. It is used…
Q: Given the following graph, what are the pKa's of the polyprotic species? Analytical Concentrations…
A: In contrast to monoprotic acids, which can only provide one proton per molecule, polyprotic acids…
Q: In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the…
A: Kool aid chromatography The blue dye, is more non-polar than the red dye and is attracted more…
Q: A mixture of dipeptides consisting of Lys-Gly, Thr-His, Ser-Leu, Glu-Gln was applied to column…
A: Ion-exchange chromatography is a type of protein purification technique in which proteins are…
Q: Biven that the reaction takes place with 100% yield, how many mi of acetic anhydride is seeded for…
A: Limiting reagent is the substances that limits the production, Here the salicyclic acid limiting…
Q: In the electrolysis of aqueous sodium bromide, there are two possible anodic reactions: *2H2O(l)…
A: Water is reduced at the cathode in the electrolysis of NaBr. This occurs because water is more…
Q: What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its…
A: Triglycerides are found in fats and oils. Fatty acyl esters join three fatty acids to glycerol to…
Q: Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into…
A: Phycocyanin is a protein-pigment complex that belongs to the family of phycobiliprotein. It is a…
Q: The data from thin layer chromatographic separation of analgesics showed ibuprofen distance to be…
A: Ibuprofen is solute here Distance travelled by Ibuprofen = 0.752 mm Distance travelled by solvent =…
Q: An amylase result is 550 U/L. A 1:4 dilution is made and reads 180 U/L. What is the reported result?
A: Enzymes act as biological catalysts that fasten reactions and increase the reaction rate by lowering…
Q: Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of…
A: Sedimentation Coefficient is the rate per unit centrifugal field experienced by the particle…
Q: The Ksp values of silver chromate Ag2CrO4 and silver iodate Ag(IO3) are given below.…
A: Given : Ag2CrO4 Ag(IO3) Ksp…
Q: Make the following prescription isotonic with lacrimal fluid: 0.06 g 0.30 g Ephedrine sulfate (E =…
A: A drug, often known as a medicine, is a chemical compound that has a biological effect on the human…
Q: The formula for a sports rub cream is given in the literature as follows: Methylsalicylate 15 mL…
A: Given that the formula for sports rub cream Methylsalicylate = 15 mLMenthol = 10 gEucalyptus oil = 1…
Q: Based on the given data, which among the lipoidal analytes is highly retarded during the development…
A: Thin-layer chromatography (TLC) is a type of chromatography that works on the adsorption principle.…
Q: Consider a HCI solution with a pH of 4.70. a) Calculate the concentration of HCl. b) Identify the…
A: pH is the scale which is used to specify the acidity or basicity of an aqueous solution. Acidic…
Q: This is a question regarding spectrophotometry. Scenario: 0.1 mL of the unknown and 0.9 mL of…
A: When 0.1 ml of the unknown and 0.9 ml of the distill water are mixed. The dilution of the unknown =…
Q: Using your stock solution of 2 M glucose, you need to prepare the following series of glucose…
A: Stock solution are the solutions of the concentrated solutions which are diluted in order to save…
Q: Explain the observed results of unboiled starch –saliva mixture with iodine solution and fehling's…
A: Starch or amylum is a polymeric sugar comprising of various glucose units joined by glycosidic…
Q: Gentiobiose has the molecular formula C12H22O11 and has been isolated from gentian root and by…
A: Disaccharides are sugars composed of 2 monosaccharide units bonded together via a O or N- glycosidic…
Q: Determine the net charge of the predominant form of Arg at (a) pH 1.0, (b) pH 5.0, (c) pH 10.5, and…
A: Arginine is an essential amino acid in juvenile humans, Arginine is a complex amino acid, often…
Q: Lactic acid 4 g Salicylic acid…
A: (a) Given that- Mass percentage of camphor (20 % w/w) This means, 100 grams solution = 20…
Q: Calculate the amount of each solution needed to make 750 mL if a dextrose 25% solution given only a…
A: Given- We make 25% dextrose of 750ml by using 60% and 5% dextrose. Use alligation method for such…
Q: Show calculations for making 10 mls each of the following dilutions of sucrose from the 0.30 M stock…
A: Dilution is defined as a term that refers to the solution that is made by adding solvent to a…
Q: Calculate the percentage efficiency of extraction of phycocyanin in sample y, if the amount of…
A: Percentage efficiency of extraction = OutputInput×100
Q: Determine the net charge of the predominant form of Asp at (c) pH 6.0, and (d) pH 11.0.
A: Introduction- Aspartic acid is an alpha non-essential amino acid that is occurring naturally in the…
Q: The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield…
A: Formula, Original cell count = #colonies / (volume plated × dilution) Viable plate count = 30 - 300
Q: If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the…
A: The Bradford protein assay is commonly used due to its sensitivity, speed, convenience, lack of a…
Q: An unknown sample was titrated with CH3COOH. After making the titration curve, no steep change was…
A: CH3COOH is a weak acid hence it will not dissociate completely in the aqueous solution to form…
Q: Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions?…
A: For using persons square we need to make a square first then on the left side of the square we write…
Q: e.2. What is the amount of each component needed, given the following media composition per liter of…
A: Introduction Lysogeny Broth (LB) Is A Nutrient-dense Media Predominantly Used For Bacterial Growth.…
Q: The decomposition reaction of atmospheric peroxyacetylnitrate (PAN) to products was studied, and the…
A: Arrhenius equation K = Ae-E/RT ln K = lnA +(-E/R)(1/T) Slope = lnK/(1/T) Y-intercept = lnA K1 =…
Q: Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the…
A: There are several tests for the qualitative analysis of carbohydrates but the Iodine test is for…
Calculate the amount of phycocyanin in Sample x in mg where A620=0.191 and A650=0.093, taking into account the dilution factor which is 100 and the total volume of extract which is 50 Note your answer to 2 decimal places.
Step by step
Solved in 2 steps
- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.192 and A650=0.090, taking into account the dilution factor (100), and the total volume of extract (45). Note your answer to 2 decimal places.Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206
- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into account the dilution factor (100), and the total volume of extract (140).Thimerosal Tincture USP contains 0.1% w/v thimerosal and 50% v/v ethyl alcohol. If the cap is left off of a 15-mL bottle of the tincture, and the ethyl alcohol evaporates leaving a final volume of 9.5 mL, what is the concentration of thimerosal in the evaporated solution expressed as a ratio strength?Tirofiban (C22H36N2O5S MWt = 440.6) is present as tirofiban HCI monohydrate MWt=495.1) at 0.281 mg/mL in a concentrated solution. (C22H36N2O5S.HCI.H₂O A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose solution and adding 50 mL of concentrated solution. Sofia who weighs weighs 87 kg requires a tirofiban dose of 0.1 mcg/kg/min for 12 to 24 hours. What would the infusion rate be (mL/h)? (Answer to 1 decimal place.)
- How many moles of EDTA (C10H16N2O3) do you have in 785µl of a 74MM EDTA solution (Mr(C10H16N203) = 292.24)? Consider all digits of your Givens significant. Round results to the appropriate number of Significant Digits. The number of moles is mmol.The standard curve provided below will allow you to answer the remaining questions. Generic Standard Curve 0.7 y = 0.9425x- 0.0034 0.6 0.5 0.4 0.3 02 0.1 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Concentration (mg/ml) - Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample.| #1: #2: -What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150? AbsorbanceTirofiban (C22H36N₂O5S MWt = 440.6) is present as tirofiban HCI monohydrate (C22H36N2O5S.HCI.H₂O MWt=495.1) at 0.281 mg/mL in a concentrated solution. A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose solution and adding 50 mL of concentrated solution. Sofia who weighs weighs 87 kg requires a tirofiban dose of 0.1 mcg/kg/min for 12 to 24 hours. What would the infusion rate be (mL/h)? (Answer to 1 decimal place.) Answer:
- Using the formula V₁D₁ = V₂D2, what volume of a 1/100 (10-2) dilution would you need to produce 100 mL of a 1/10000 (10-4) dilution? (Note: provide your answer in decimal format to three decimal places.)The Ksp values of silver chromate Ag2CrO4 and silver iodate Ag(IO3) are given below. Ag2CrO4 Ag(IO3) Ksp 1.12 x 10-12 3.17 x 10-8 Based on these Ksp values, which of the following is true? Choose one option only. Options: a. In the solution consisting of 1.00 x10-4 M Ag+ and 5.00 x10-5 M CrO42-, Ag2CrO4 precipitate will form. b. In the solution consisting of 1.0 x10-4 M Ag+ and 1.0 x10-4 M IO3-, Ag(IO3) precipitate will form. c. In pure water, the solubility of Ag2CrO4 is lower than the solubility of Ag(IO3). d. In the solution consisting of 0.200 M CrO42- and 0.200 M IO3-, Ag2(CrO4) will precipitate first if we add Ag+ ions gradually into the above mixture.Tirofiban (C22H36N₂O5S MWt = 440.6) is present as tirofiban HCI monohydrate MWt=495.1) at 0.281 mg/mL in a concentrated solution. (C22H36N2O5S.HCI.H₂O A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose s and adding 50 mL of concentrated solution. Sonya who weighs weighs 60 kg requires a tirofiban dose of 0.4 mcg/kg/min for 30 minute- What volume would remain after 30 minutes? Answer: 94.53 mL X The correct answer is: 236 mL