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Calculate the molar concentration of a purified enzyme (MW 25000 Dal) in a solution of 0.10 g/ml of protein.

Calculate the molar concentration of a purified enzyme (MW 25000 Dal) in a solution of 0.10 g/ml of protein.

Introduction to General, Organic and Biochemistry
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter4: Chemical Reactions
Section: Chapter Questions
Problem 4.33P: 4-33 When a solution of hydrochloric acid, HCl, is added to a solution of sodium sulfite, Na.2S03,...
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Calculate the molar concentration of a purified enzyme (MW 25000 Dal) in a solution of 0.10 g/ml of protein. Choose the dilution series to make a 1/300 dilution of a dye, 1.0 ml final volume is needed: (a) 1. 100 µl dye mixed with 900 μl H₂O, 2. 100 μl dilution 1 mixed with 900 μl H₂O, 3.400 μl dilution 2 mixed with 800 μl H₂O: then take 1.0 ml of dilution 3. (b) 1. 50 μl dye mixed with 950 μl H₂O, 2. 100 μl dilution 1 mixed with 1400 µl H₂O: then take 1.0 ml of dilution 2. (c) 1. 40 μl dye mixed with 960 μl H₂O, 2. 100 μl dilution 1 mixed with 1100 μl H₂O: then take 1.0 ml of dilution 2.
Transcribed Image Text:Calculate the molar concentration of a purified enzyme (MW 25000 Dal) in a solution of 0.10 g/ml of protein. Choose the dilution series to make a 1/300 dilution of a dye, 1.0 ml final volume is needed: (a) 1. 100 µl dye mixed with 900 μl H₂O, 2. 100 μl dilution 1 mixed with 900 μl H₂O, 3.400 μl dilution 2 mixed with 800 μl H₂O: then take 1.0 ml of dilution 3. (b) 1. 50 μl dye mixed with 950 μl H₂O, 2. 100 μl dilution 1 mixed with 1400 µl H₂O: then take 1.0 ml of dilution 2. (c) 1. 40 μl dye mixed with 960 μl H₂O, 2. 100 μl dilution 1 mixed with 1100 μl H₂O: then take 1.0 ml of dilution 2.
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