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- Can sucrose pass freely through the membrane of a dialysis tube? Explain using examples. (Biochemistry)Can water pass freely through the membrane of a dialysis tube? Explain. (Biochem)Inside a beaker, dialysis membrane A is filled with a 50% glucose solution and dialysis membrane B is filled with a 50% NaCl solution. Both bags are put into beakers containing 100% water. Which of the following choices is correct? (hint: the dialysis membrane is only permeable to water) a) For both solutions, it is isosmotic, and hypotonic so water flows into the membrane.b) For both solutions, it is isosmotic, and isotonic so there is not net flow of waterc) For both solutions, it is hyposmotic, and hypotonic so water flows into the membraned) For both solutions, it is hyperosmotic, and hypertonic so water flows into the membranee) For both solutions, it is hyposmotic, and hypertonic so water flows out of the membrane
- Osmosis is often defined as the flow of water through a semipermeable membrane, from a dilute solution to a more concentrated solution. Video experiment #1 – saturated sucrose/dialysis tubing/water osmosis from U Mich. https://youtu.be/pCupvFGN4bw A saturated solution of sucrose-containing a red dye is separated from water by dialysis material. What happens to the volume of sucrose solution during the experiment? How do you know that the sucrose solution did not flow into the beaker of water? (Give evidence from your observation of the experiment.) Diffusion, Osmosis, Dialysis video: https://youtu.be/tHzkRtzVmUMDialysis, the flow of both solvent and small molecules or ions through a dialyzing membrane, is important clinically in the operation of the artificial kidney machine.If a dialysis bag containing 1% sucrose is placed in a beaker containing 25% sucrose, what would you expect to happen? (hint: only water can move in and out os the bag through osmosis, sucrose is too large; this is a hypertonic environment) A) there will be a loss in weight of the bag due to water leaving the bag B) there will be a gain in weight of the bag due to water moving into the bag C) the weight of the bag will remain the same, with water moving in both directions equally D) sucrose will break down and will move into the bag, increasing the weight of the baga) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings. b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings. c) Based on Table 1, calculate the glucose concentration / 1 gram tumour cells in conditions A, B and C. Show your workings.
- If there was India Ink added to a culture of Paramecium caudatum, it would increase the average number of food vacuoles because India ink contains no sugar or nutrients and the formation of food vacuoles would be the first stage in the metabolic process when digesting nutrient-rich particles. If Sugars (sucrose or mannitol) were added to this culture to modify extracellular solute concentration, what effect would we see in the microscopic in regards to the number of food vacuoles? Could sugars have other effects beyond altering solute concentration? Be metabolized?If there was India Ink added to a culture of Paramecium caudatum, it would increase the average number of food vacuoles because India ink contains no sugar or nutrients and the formation of food vacuoles would be the first stage in the metabolic process when digesting nutrient-rich particles. If Sugars (sucrose or mannitol) were added to this culture to modify extracellular solute concentration, what effect would we see in the microscopic in regards to the number of food vacuoles and will the amount of food vacuoles change over time? Could sugars have other effects beyond altering solute concentration? Be metabolized?Dialysis is a process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. What are the factors affecting dialysis.
- 1) Cystic fibrosis is due to a defect in a pump of the cell membrane. Discuss the causes and treatments of this disease.A dialysis tube is constructed of a cellophane-like material. The molecular structure of the material forms a membrane containing numerous small-diameter pores. The dialysis tube containing a 10% protein solution and 10% amino acid solution was placed into a beaker containing water. After 10 minutes the water in the beaker tested positive for amino acids but negative for protein. Why were amino acids but not protein able to pass through the dialysis membrane? Water molecules blocked the pores so that the protein was unable to pass through the membrane. Amino acids, but not proteins, are small enough to pass through the pores in the tube's membrane. Protein is too hydrophobic to pass through the pores in the membrane. Amino acids blocked the pores so that the protein was unable to pass through the membrane.How are osmosis and dialysis similar? How do they differ?