Carbonic anhydrase of erythrocytes (Mr 30,000) has one of the highest turnover numbers known. It catalyzes the reversiblehydration of CO₂.H₂O + CO₂2H₂CO3This is an important process in the transport of CO₂ from the tissues to the lungs. If 5 µg of pure carbonic anhydrase catalyzesthe hydration of 0.35 g of CO₂ in 1 min at 37 °C at Vmax, what is the turnover number (kcat) of carbonic anhydrase?Kcat2.0466 ×107Incorrectmin-1

The value of Kcat represents the catalytic efficiency of an enzyme. It is also known as the turnover…

Answered: Carbonic anhydrase of erythrocytes (M,…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 16P: Energetics of the Hexokinase Reaction The standard-state free energy change. Gfor the hexokinase...

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Understanding the Mechanism of Hemolytic Anemia Genetic defects in glycolytic enzymes can have serious consequences for humans For example, defects in the gene for pyruvate kinase can result in a condition known as hemolytic anemia. Consult a reference to learn about hemolytic anemia, and discuss why such genetic defects lead to this condition.
Energetics of the Hexokinase Reaction The standard-state free energy change. Gfor the hexokinase reaction, is â€” 1 6.7 kJ/mol. Use the values in Table I to calculate the value of Gfor this reaction in the erythrocyte at 37Â°C.
Acid phosphatases are an important group of enzymes that can be detected in human bloodserum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyzebiological phosphate esters as follows:R-O-P-O3-2 + H2O -----> R-OH + HO-P-O3-2Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver,and prostrate gland. The enzyme from the prostrate gland is clinically important because anincreased activity in the blood is frequently an indication of cancer of the prostrate gland.Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acidphosphatases from other tissues. How can you use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostrate gland in humanblood serum?
Hemoglobin molecules exposed to high levels of glucose areconverted to glycated products. The most common, referred to as hemoglobin A1C (HbA1C), contains a b-chain glycatedadduct. Because red blood cells last about 3 months, HbA1Cconcentration is a useful measure of a patient’s blood sugarcontrol. In general terms, describe why and how HbA1Cforms.
Carbonic anhydrase catalyzes the hydration of CO. CO2 + H2O ¬ H½CO3 The Km of this enzyme for CO, is 1.20×104 µ.M. When [CO,] = 3.60×104 µM, the rate of reaction was 4.50 umol·mL! sec-1 a What is Vmax for this enzyme? umol·mL-!sec-!
If "P-labeled inorganic phosphate were introduced to erythrocytes undergoing glycolysis, would you detect "P in the glycolytic intermediates? Suppose the results are positive, explain the relevant reaction(s) and the incorporation of P that you would observe.
In an erythrocyte undergoing glycolysis, what would happen if there is a sudden increase in the concentration of (i) AMP (ii) Fructose 2,6 bisphosphate
Human blood serum contains a class of enzymes known as acid phosphatases, whichhydrolyze biological phosphate esters under slightly acidic conditions (pH 5.0): Acidphosphatases are produced by erythrocytes, the liver, kidney, spleen, and prostate gland. The enzyme of the prostate gland is clinically important, because its increased activity in the bloodcan be an indication of prostate cancer. The phosphatase from the prostate gland is stronglyinhibited by tartrate ion, but acid phosphatases from other tissues are not. How can thisinformation be used to develop a specific procedure for measuring the activity of the acidphosphatase of the prostate gland in human blood serum?
This is the kcat question. At maximum saturation, 28.3µg of enzyme in 25mL water catalyzes the oxidation of ethanol at a rate of 2.5mM/min. Calculate kcat in units of seconds if the enzyme has a molar mass of 65kg/mol. The more correct work you show, the more credit you will get.
calculate the actual free energy of hydrolysis of ATP, delta Gp in the erythrocytes of a new species. The standard free-energy of hydrolysis of ATP is also -30.5kJ/mol in this species, but the concentrations in this specie's erythrocytes are 0.00775 mM ATP, 0.00027 mM ADP and 0.00418 mM Pi. Assume the pH is 7.0 and the body temperature of this species is 43.0oC. Calculate your answer as kJ/mol to two decimal places.
Consumption of nitrate-rich vegetables such as spinach have been shown to reduce blood pressure in prehypertensive individuals (i.e., those having no history of clinical hypertension), a phenomenon attributed to increased nitric oxide synthesis. Describe the reactions that convert nitrate (NO3 −) to nitric oxide (NO•) in the body.
A 42 yr old woman is not in shape but still decides to run a marathon. During the event, she collapses and is brought to the ER. Arterial blood gas analysis shows PO2 of 100 mmHg (normal), PCO2 of 42 mmHg (normal), and pH of 7.30 (low). Further evaluation reveals high anion-gap metabolic acidosis. Increased activity of which of the following biochemical pathways is the basis of the clinical findings? a. ETC b. Citric acid cycle c. Anaerobic glycolysis d. Beta-oxidation of fatty acids e. Mitochondrial oxidative phosphorylation

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