Q: Explain why exome sequencing can be almost as valuable as genome sequencing.
A: Whole genome sequencing refers to technique which used to analyze complete genome of an organism at…
Q: Describe the shotgun method for sequencing an entire genome.
A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…
Q: I am having issues with differentiating between the different types of PCRs and why the different…
A: The polymerase chain reaction (PCR) is a technique in which several million copies of the desired…
Q: Complete the flowchart about the tools and processes used in genetics and biotechnology. These terms…
A: DNA fingerprinting is a technique used to identify the short tandem repeats of DNA. DNA…
Q: What is exome sequencing used for?
A: DNA sequencing: It is a method to find a sequence of nucleotides by using the DNA sequencing…
Q: Explain Proofreading by DNA polymerase.
A: A DNA polymerase is an enzyme that catalyze the synthesis of DNA molecules from nucleoside…
Q: Describe the technology behind identifying, synthesizing,sequencing, and amplifying DNA.
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: How does a DNA microarray work?
A: Gene microarray technology enables to deposit many different DNA sequences on a small glass slide,…
Q: Define genetic engineering and biotechnology, and summarize theimportant purposes of these fields.…
A: Manipulation of nucleic acids has allowed us to identify organisms and their genes, and use those…
Q: PCR?
A: PCR: Polymerase chain reaction PCR is the technique of modern molecular biology labs, it is a bio…
Q: Briefl y summarize the steps involved in DNA sequencing and describe its applications.
A: DNA Sequencing is the process or method which determines the order of the four nucleotides bases…
Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?
A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…
Q: Compare tblastx and tblastn software packages of genome sequence analysis.
A: BLAST stands for Basic Local Alignment Search Tool •What makes BLAST so popular??- Good balance of…
Q: What is exome sequencing ? Why it is important ?
A: The exome has historically been described as the sequence in the genome that includes all exons of…
Q: Explain how DNA microarrays are used in the study ofgenomics.
A: DNA (deoxyribonucleic acid) is double helix, coil structure that is responsible for transfer of…
Q: Briefly summarize the steps involved in DNA sequencing anddescribe its applications.
A: DNA sequencing is a technique that is used to determine the correct sequence of DNA by a series of…
Q: How to use a microarray for transcriptome analysis ?
A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…
Q: Describe a high-throughput, automated method bywhich millions of DNA templates may be…
A: The process of determining the exact order in which the sequence of the nucleotides are present in…
Q: Define about computer-automated high-throughput DNA sequencing ?
A: DNA sequencing is a commonly used method after the gel electrophoresis to determine the order of…
Q: what is the difference between genomics and transiptomics
A: The branch of biology that deals with the study of genes, variations in genes, and heredity process…
Q: What are the advantages of Next Generation Sequencing?
A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…
Q: What are the advantages of doing DNA sequencing?
A:
Q: Give a step by step method and diagram of how reversible terminator sequencing works.
A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…
Q: Discuss the principles , uses, advantages and disadvantages of illumina sequencing method
A: DNA copies obtained from PCR is subjected for gene sequencing through different gene sequencing…
Q: Explain the relationship among the following terms: genomics, proteomics, gene, protein, genotype,…
A: Introduction :- Genomics is the study of an organism's entire genome, which includes genetic…
Q: What is the difference between whole exome and whole genome sequencing?
A:
Q: Summarize the steps involved in sequencing a protein.
A: Protein sequencing is a process, which is comprised of amino acid sequencing of all the parts of the…
Q: What is gene sequencing used for?
A: DNA sequencing is the process of determining the sequence of nucleotide bases that make up a DNA.…
Q: Describe how Restriction Enzymes, Plasmids, DNA Hybridization, and PCR can be used for molecular…
A:
Q: What are the main differences between whole genome sequencing and whole exome sequencing?
A: whole genome sequencing is sequencing the entire genome of the organism, where as whole exome…
Q: Define the following terms:a. PCRb. DNA microarrayc. chromosomal jumpingd. genome projecte.…
A: Since you have posted a question with multiple sub-parts, we will solve the first three subparts for…
Q: Explain the difference between a DNA profile and a microrarray.
A: Deoxyribonucleic acid (DNA) analysis is an important biotechnological tool that has vast…
Q: Why amplify DNA using PCR before doing electrophoresis? Why not just take your DNA sample and…
A: The polymerase chain reaction (PCR) is a typical laboratory technique for making multiple copies of…
Q: Define the terms DNA profiling and DNA sequencing.
A: DNA profiling and DNA fingerprinting are two molecular approaches for identifying individuals based…
Q: Hi, I would like to know which program is used for the graphical presentation of the results of a…
A: A genome wide linkage association study (GWAS) is an approach used in genetic research to associate…
Q: Compare the knowledge obtained from geneticdissection and from genome sequencing.
A: The term genome refers to the entire genetic material of an organism. Human genomes have about 3…
Q: Provide five advantages of Next Generation Sequencing? and explain each of these advantages.
A: Here I will discuss about the advantages of next generation sequencing.
Q: How to Develop new sequencing technologies, such as highthroughputcomputer-automated sequencers, to…
A: Sequencing technologies is the method used to sequence the genome or the fragment of DNA. Earlier…
Q: gun sequencing ncing every gene is mapped then
A: Shotgun sequencing is a method used for DNA sequencing. It is carried out by breaking the DNA…
Q: Discuss the principles,uses , advantages and disadvantages of SMART sequencing.
A: Single-molecule real-time sequencing is a parallelized single-molecule DNA sequencing method.…
Q: What is a n automated DNA sequencing?
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: Explain the use of Annotation to Identify Gene Sequences ?
A: The attachment of biological information to the gene sequence is known as genome annotations. Three…
Q: List 3 more applications of Recombinant DNA technology.
A: Recombinant DNA technology is the joining together of DNA molecules from two different species and…
Q: What can exome sequencing do for you?
A: The sequencing of the nucleic acid is the process through which the exact sequence of the nucleotide…
Q: Compare and contrast the assembly of genomes using Sanger and next-generation sequencing (NGS)…
A: Genome sequencing: genome means total gene content present in the organism and sequence means to…
Q: Discuss the similarities and differences between the methods used in genomics and transcriptomics.
A: Genome sequencing is often regarded as decoding. Genomics is the study of genomes through analysis,…
Q: Define about whole-genome sequencing ?
A: There may be millions of genes present in the organisms. It is not possible to sequencing all types…
Q: What is single molecule sequencing in real time (SMRT) ?
A: DNA sequencing is a technique for determining the order of the four nucleotide bases found in DNA:…
Chromosomal microarray, CGH, and Exome sequencing: compare and contrast!
Step by step
Solved in 4 steps
- What molecule is next generation sequencing analyzing? DNA, RNA, or protein.Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research. Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity. The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c. The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp). Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research. Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity. The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c. The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp). Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…