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Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Create a descriptive introduction about compound microscope. Cite your reference/s if possible.
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- Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)Write the steps for setting up a microscope for Kohler illumination. The first step has been written for you. 1 . Focus the microscope on a section.Using the provided materials and the microscope itself, review the parts of the microscope including the name, where it is located, its function and the appropriate times to use. Record your observations and notes as vou will require this information when writing yourlab report
- How does the compound microscope achieve magnification? resolution? Cite your reference/s if possible.Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.Give a particular use/uses of the following types of a microscope: 1. Research microscope 2. Stereomicroscope 3. Student microscope 4. Projection microscope
- can you help me to identify by labelling the slidesWhat is the viewing field diameter when you use 10X magnification on the dissecting microscopeWhat are the corresponding ring colors of the objective lenses of the microscope? Scanner _________________________ Low Power Objective (LPO) _________________________ High Power Objective (HPO) _________________________ Oil immersion Objective (OIO) _________________________
- Help withidentify the parts and functions of a microscope. Provide your answers on the spaces provided below. Fine Focus ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Coarse Focus ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Stage Clip ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Rack Stop…Table 1. Objective Lens Scanner Low Power Objective High Power Objective Oil Immersion Objective Total Magnification Objective magnification Ocular magnification Procedure: 1. Locate and record the numbered X in the ocular and the objectives. These numbers represent the magnifying ability of the microscope lenses 2. To compute the magnification, get the product of the magnifying power of the objectives and the eyepiece. Reflect your results in Table 1.3