CRISPR-cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below options are on the right

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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CRISPR-cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below

options are on the right 

Design a set of targeting sequences using
algorithms.
Prompts
Submitted Answers
Stage 1:
Design a set of targeting sequences using algorithms.
O Purify plasmids
Stage 2:
Clonal isolation
O Synthesise DNA insert oligonucleotides
Stage 3:
Transfect cells
Clonal isolation
Stage 4:
Synthesise DNA insert oligonucleotides
Clonal characterisation (analysis of phenotype)
Stage 5:
Purify plasmids
Sequence
Stage 6:
Sequence
Transfect cells
Stage 7:
Clonal characterisation (analysis of phenotype)
Add green fluorescent protein gene sequence into
plasmid to aid selection of correctly transfected
Stage 8:
Select for correctly gene edited cells (using antibiotic
resistance and/or colour production for example)
cells.
Stage 9:
Add green fluorescent protein gene sequence into
Clone into CRISPR-cas9 expression vector
plasmid to aid selection of correctly transfected cells.
Select for correctly gene edited cells (using
antibiotic resistance and/or colour production for
example)
The due date for this submission has passed. New attempts will be marked as late.
Last sav
Transcribed Image Text:Design a set of targeting sequences using algorithms. Prompts Submitted Answers Stage 1: Design a set of targeting sequences using algorithms. O Purify plasmids Stage 2: Clonal isolation O Synthesise DNA insert oligonucleotides Stage 3: Transfect cells Clonal isolation Stage 4: Synthesise DNA insert oligonucleotides Clonal characterisation (analysis of phenotype) Stage 5: Purify plasmids Sequence Stage 6: Sequence Transfect cells Stage 7: Clonal characterisation (analysis of phenotype) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected Stage 8: Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) cells. Stage 9: Add green fluorescent protein gene sequence into Clone into CRISPR-cas9 expression vector plasmid to aid selection of correctly transfected cells. Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) The due date for this submission has passed. New attempts will be marked as late. Last sav
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