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Q2. Study the diagram given below and write description for each part.
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- b. Cleavage with chymotrypsin produces the following fragments: Band A: CN , NLQY, GIVEQCCHKRSEY Band B: F, Y, DPTKM, IACCVRGF, RTTGHLCGKDLVNALY Cleavage with Staphilococcus aureus V8 protease produces the following fragments: Band A: GIVE, YNLQNYCN, QCCHKRCSE Band B: PTKM, RTTGHLCGKD, LVNALYIACGVRGFFYD What is the amino acid sequence of the protein? Type your responsecomplete the sentence. In the Sanger method, the carbon atom of the reagent fluorodinitrobenzene acts as an , which is attacked by the nitrogen from the tripeptide (give the name of the tri-peptide using the one-letter-codes of the 3 amino acids forming it, no spaces or punctuation between letters. eg. ABC) shown above.B. Enumerate all the possible DNA nucleotide base sequence for the amino acids given. 4. Met – Leu – Ala - Gly- Glu - Gly- Gln- Glu- Ala - Ala - Pro - Leu
- MATCH THE FF. WITH THE CHOICES BELOW nitrosoamines nitrosoguanidines nitrosoureas alkyl sulfates nitrogen mustards 5-bromouracil 2-amino purine adenine to guanine adenine to cytosine cytosine to thymine CHOICES: Transition mutation Transversion mutation Alkylating agents Base analogExplain the advantage of using Fluorescence Recovery After Photobleaching (FRAP) in determining protein localisation I notes = Notes Comments MAY 7.Arial BIUA 11 + .. | I 1 I 3 I 4 i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ Inone +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why? MacBook Air 000
- Mutated DNA Sequence #2 T A C G A C C T T G G C G A C G A C T … What’s the mRNA sequence? (Circle the change) What will be the amino acid sequence? Will there likely be effects? What type of mutation is this? ________________________________Elaborate on the abbreviation RTP. What does it stand for? What is it usedfor?o-H HN-H H. Give the full amino acid name, three-letter name and one-letter names of the amino acids in the oligopeptide starting from the amino terminal to the carboxyl terminal. Make sure that the amino acid names are arranged in CORRÉCT order