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- Discuss the different methods of water analysis to determine pollution.List down two important precautions in conducting brine solution experiment.Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 10 cfu’s in the 1:100 plate and 1 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample?
- Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 50 cfu’s in the 1:100 plate and 3 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample? Is water acceptable for potable or Recreational use? Explain. When do you use filtration rather than serial dilutionWhat are the advantages and disadvantages of the filter method in the analysis of water samples? Why is the incubation temperature critical for this test?What are the expected problems in the centrifuge in case of imbalance of samples ? *
- From a water sample, you must determine the number of colonies present (CFU's/ml.). However, looking at the plates of the dilutions you find some cultures with scattered colonies. Briefly explain the reason for eliminating or not considering these plates in the data collection. Short answer please..Differentiate individual sampling and pooled sampling in dissolution.Describe the general requirements of biological buffers for an effective experiment.
- Define water activity and briefly describe how it can be determined. Why is it difficult for microorganisms to grow at low aw values?Is it safe to use 100g of Lye (NaOH) as a disinfectant at homes? Justify your claim by computing the percent composition. Claim: Yes/No Solution:Which are the toxic chemicals used in preparation of samples for TEM? How should these be handled safely?