DNA fingerprinting uses restriction enzymes to identify a person or organism based on the pattern of DNA fragments. What is the characteristic that is the basis for DNA fingerprinting studies? O DNA is present in all body tissues of an organism. All DNA strands can be digested using restriction enzymes. The DNA of individuals within a species is exactly the same. O The pattern of DNA fragments varies from person to person.

DNA fingerprinting uses restriction enzymes to identify a person or organism based on the pattern of DNA fragments. What is the characteristic that is the basis for DNA fingerprinting studies? O DNA is present in all body tissues of an organism. All DNA strands can be digested using restriction enzymes. The DNA of individuals within a species is exactly the same. O The pattern of DNA fragments varies from person to person.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 10QP: Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands...

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DNA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed. Gel Electrophoresis Procedure The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells. An electric current is passed through the gel. DNA fragments are treated with a dye. A restriction endonuclease is added to the DNA. Using micropipettes, the DNA samples are added to the wells. DNA fingerprint is produced. DNA fragments are produced. The order in which a DNA fingerprint is produced using gel electrophoresis is Answer, Answer, Answer, Answer, Answer, Answer, and Answer.
1 2 Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will model the process yourself to solve a crime. Good luck, detective! Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA is shown in Figure 5. 10 50 DNA Profile from Forehead Print Number of base pairs 00 50 40 D58818 075830 I 16 MU DES1179 Shandand (10) 70 CSF1PO DITS820 80 100 Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179). and data for the four STR loci that were included in the A suspect was identified in the case. Her DNA…
What purpose do short DNA primers serve in PCR? O They determine which sequence of DNA is copied O They synthesize new DNA O They synthesize proteins O They separate RNA from DNA MacBook Air 80 888 F7 F6
Which of the following statements is/are true? Choose only the best answer. Repetitive DNA can influence the appearance of an organism. Simple sequence DNA, transposable elements, and large-segment duplications are examples of repetitive DNA. Repetitive DNA can be useful in genetic profiling. All three of the other statements are true.
The segment of DNA shown in the figure has restriction sites I and II, which create DNA restriction fragments A, B, and C. Which of the gels produced by electrophoresis best represents the separation and identity of these fragments? Reminder: a positive electrode is located at one end of the gel and the negative electrode at the other end. II O a. From the positive to the negative end, the order of the fragments will be B, A, C O b. From the positive to the negative end, the order of the fragments will be A, B, C O c. From the negative to the positive end, the order of the fragments will be B, A, C O d. From the positive to the negative end, the order of the fragments will be C, A, B O e. From the negative to the positive end, the order of the fragments will be A, B, C
Why is DNA polymerase a required part of PCR? O It synthesizes new DNA O It synthesizes proteins O It degrades the template DNA O It separates RNA from DNA MacBook Air
Which is the correct order of the PCR reaction? Annealing of primers, melting of DNA strands, and extension of new strand Melting of DNA strands, extension of new strand, annealing of primers O Melting of DNA strands, annealing of primers, extension of new strand O Extension of new strand, annealing of primers, melting of DNA strands O Annealing of primers, extension of new strands, melting of DNA strands
Which ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt rubbing alcohols detergent solution water What is the purpose of PCR? To copy and then make many copies of a specific region of DNA To copy the entire human genome To make just a few copies of DNA To reveal the sequence of a piece of DNA If enzyme catalase has an optimum pH of 7.0, What do you think will happen en the pH is lowered to 3.0? The rate of its enzyme action is increased. Its enzyme action is not affected at all. The rate of its enzyme action is decreased. Its enzyme action ceases or stops.
Why is Taq polymerase used in a polymerase chain reaction (PCR)? O Unlike other DNA polymerases, Taq polymerase is heat stable and survives the 94 degree denaturation step in PCR. Taq polymerase is more efficient than other DNA polymerases. O Taq polymerase can produce DNA from an RNA template, unlike other DNA polymerases. O Taq polymerase has proofreading activity and thus makes fewer errors than other DNA polymerases. Question 44 Which of the following statements about the differences between prokaryotic and eukaryotic genomes is FALSE? O Prokaryotic genomes have centromeres, whereas eukaryotic genomes do not. O Eukaryotic genomes have telomeres, whereas prokaryotic genomes do not. Prokaryotic genomes have one origin of replication, whereas eukaryotic genomes have multiple origins of replication. O Prokaryotic genomes are less complex than eukaryotic genomes.
Humans have very similar DNA sequences, with approximately 999/1000 letters being ide One possible way of telling the difference between these very similar sequences is by: Genetic engineering Analyzing RFLPS using gel electrophoresis Comparing the total amount of DNA from two or more people
An important feature of restriction enzymes is that each enzyme only recognizes a specific palindrome and cuts the DNA only at that specific sequence of bases. A palindromic sequence can be repeated a number of times on a strand of DNA, and the specific restriction enzyme will cut all those palindromes, no matter what species the DNA comes from. A linear DNA molecule is represented below. The DNA is represented by one line, although in actuality, DNA has two strands. If the DNA molecule has two restriction sites, specifically two repeats of a specific palindrome sequence, A and B, for a specific restriction enzyme: How many fragments would be produced if the DNA is cut by that enzyme? Number each fragment Which fragment would be the largest? Which fragment would be the smallest?
Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase