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Enumerate and describe the signs of emulsion instability. At least 5-7
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- The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…Acetaminophen or paracetamol, the active ingredient in many pain killers absorbs in the UV range with a λmax at 250 nm in neutral solutions (methanol usually). Its extinction coefficient is 14 mM-1cm-1. Each commercially available tablet (Tylenol, Panadol or Herron) contains 500 mg paracetamol. Its molecular weight is 151.1. Using this information, calculate the dilution required to analyse the concentration of paracetamol in a commercially available paracetamol tablet. Start by dissolving the tablet in 100 mL methanol. Show working out.A compound Y is to be determined by UV-visible spectrophotometry. A calibration curve is constructed from standard solutions of Y with the following results: 0.20 ppm, A = 0.870; 0.40 ppm, A = 0.840; 0.60 ppm, A = 0.800; 0.80 ppm, A = 0.750; 1.00 ppm, A = 0.700; 1.50 ppm, A = 0.620; and 2.00 ppm, A = 0.530. a. What is the equation of the calibration curve for compound Y? b. What is the linearity of the calibration curve for compound Y?
- (4) Below are depictions of a student's TLC plates in which fractions 1 - 10 from their column are spotted. Lane 1 2 3 4 5 67 8 9 10 (a) Which fractions contain pure fluorenone? Which contain pure ferrocene? (b) Which lanes, if any, represent mixed fractions? (c) Would the student incorporate lanes 6 and 7 into an Erlenmeyer flask of a combined fraction? Explain with 10 words or less.7-21. Calculate the NBOD of 200 mg/L of a casein (C8H₁2O3N₂) dairy waste. Assume the following reactions: C8H₁2O3N₂ + 80₂ = 8CO₂ + 3H₂O + 2NH3 NO3 + H+ + H₂0 NH3 + 20₂ 7-22. Derive an expression for the final temperature T. of the mixture ofPe Identity Molecu tr, min w, min T (oC) Chart ak ler area weight (cm2) 1 Air peak small Tm=0, small 58 50 2 CH31 141,9 6,60 0,55 100 13,0 3 C₂H5l 156,0 9,82 1,00 127 12,0 4 ISO.C3H7I 170,0 11,90 1,04 139 10,0 5 n.C3H7I 170,0 13,04 1,08 148 7,2 6 CH2l2 261,8 19,10 1,60 193 8,0 8. A mixture of five alkyl iodides is separated by gas chromatography using a column packed with crushed five brick cooled with silicone oil. The column is so heated that its temperature rises linearly during the separation. What is the resolution of peaks: a. 2 & 3 b. 4 & 5 C. 5 & 6
- 3-23 Complete this table for H,O: T, °C P, kPa v, m³/kg Phase description 140 0.05 550 Saturated liquid 125 750 500 0.140h) i) j) Ph + НО OSI(CH3)3 Br PPH3 1) "BuLi /THF/rt / 1.5 h H CH3 homo /rt / 1 h 2) -Si(CH3)3 OCH3 TiCl4 CH₂Cl₂/-78°C / 15 min PPh3/1₂ (1 eq) imidazole / THE 0°C-itA compound X is to be determined by UV-visible spectrophotometry. A calibration curve is constructed from standard solutions of X with the following results: 0.50 ppm, A = 0.24; 1.5 ppm, A = 0.36; 2.5 ppm, A = 0.44; 3.5 ppm, A = 0.59; 4.5 ppm, A = 0.70. A solution of unknown X concentration had an absorbance of A = 0.50. Find the slope and in te rcept of the calibration curve, the standard error in Y, the concentration of the solution of unknown X concentrat ion, and the standard deviation in the concentration of X. Construct a plot of the calibration curve and determine the unknown concentra tion by hand from the plot. Compare it to that obtained from the regression line.
- 1) The ratio strength of a 10mL vial of neostigmine methylsulfate injection USP is 1:1500. Calculate the amount of neostigmine methylsulfate in milligrams contained in the 10mL vial. (Round to the whole number).Describe the primary and secondary criteria for having achieved V ̇O2 max.A compound X is to be determined by UV/Visible spectrophotometry. A calibration curve is constructed from standard solutions of X with the following results: 0.50 ppm, A =0.24; 1.5 ppm, A =0.36; 2.5 ppm, A = 0.44; 3.5 ppm, A = 0.59; 4.5 ppm, A =0.70. Find the slope and intercept of the calibration curve, the standard error in y, the concentration of the solution of unknown X concentration and the standard deviation in the concentration of X. Construct a plot of the calibration curve and determine the unknown concentration by hand from the plot (only for the calibration curve, slope and y-intercept. Make an equation on how to solve for the concentration of unknown).