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- Define the principle immunoturbidimetric C3 assayAs everyone is aware of, suspected COVID-19 patients are being diagnosed using the Nasopharyngeal Swab Test through Reverse-Transcriptase Polymerase Chain Reaction. Explain its principle; its advantages and disadvantages.Describe three examples of how antibodies are used as reagents in cell biology and molecular biology.
- In relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?In relation to immunotechnology, answer the following: A- Answer the following in relation to Hybridoma technology: → What is the cancer cell line name used in hybridoma technology? → Give an example of murine Monocolonal Antibody produced by hybridoma technology? B- Give ONE main difference between: Murine MABs and fully human MABs? and explain the reason behind developing different forms of Monoclonal antibodies (chimeric MABs, humanized MABs, and fully human MABs) C- You are in an internship in one major Hospital, You were in rotation with a clinical pharmacist in oncology section and you came over a breast cancer patient. The personal doctor was making a quick visit to the patient to make sure the patient is following the therapeutic protocol and the treatment is running smoothly. The doctor wrote in the prescription sheet “Trastuzumab” to be dispensed by the In Patient Pharmacy, when the clinical pharmacist saw this he advised the doctor politely to use the…Outline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.
- EIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure usedto confirm screening tests that are positive for humanimmunodeficiency virus (HIV)?Explain the steps involved in monoclonal antibody production using hybridoma technology.Explain the difference between in vivo and ex vivogene therapy.
- What is the application of antibodies in biotechnology? Please answer at your own words.Biochemistry techniques: a) Name and briefly describe the ELISA assay with greater sensitivity for detection of the viral infection. Focus on antigens and antibodies. Justify your reasoning. B) Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa, Arrange the molecules in order of their elution from a gel filtration column. Elutes first (A) to elute last (D) arrangement below: A) B) C) D) Explain your reasoning . Instructor C). What characteristics determine the position of a protein at the end of isoelectric focusing of 2D SDS- PAGE?How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..