Find the following: 1. slope and intercept of the calibration curve 2. the mean concentration of the solution of unknown X concentration if the absorbance of 3 replicates are A = 0.29, A = 0.30 and A = 0.32. 3. the standard deviation in the concentration of X

Find the following: 1. slope and intercept of the calibration curve 2. the mean concentration of the solution of unknown X concentration if the absorbance of 3 replicates are A = 0.29, A = 0.30 and A = 0.32. 3. the standard deviation in the concentration of X

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.17QAP: Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC...

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Question

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A compound X is to be determined by UV/visible spectrophotometry. A calibration curve is constructed from standard solutions of X with the following results:

0.50 ppm, A = 0.24;

1.5 ppm, A = 0.36;

2.5 ppm, A = 0.44;

3.5 ppm, A = 0.59; and

4.5 ppm, A = 0.70.

Find the following:

1. slope and intercept of the calibration curve

2. the mean concentration of the solution of unknown X concentration if the absorbance of 3 replicates are A = 0.29, A = 0.30 and A = 0.32.

3. the standard deviation in the concentration of X

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