How can single-cell genomics be used to addressmicrobial dark matter?

Q: Explain how are the physiological characteristics of bacteria identified using biochemical test?

A: The physiological properties that are used for the identification of bacteria includes the nutrient…

Q: what is gel electrophoresis

A: Question : What is Gel electrophoresis,?

Q: What is the methods used for identifying unknown bacteria?

A: Ability of identifying various microorganisms have many important application.For example,in the…

Q: How VNTRs are useful for DNA profiling ?

A: DNA profiling is defined as technique which involves specific DNA patterns known as profiles…

Q: What is the difference between complete medium and minimal medium? How are complete media and…

A: Nutritional requirements of bacteria and the culture media are defined by microbiologists. The…

Q: What is VNTR profiling, and what are the applications of thistechnique?

A: DNA profiling is a forensic technique in criminal investigations. It is used as a person's DNA to…

Q: Why is pure culture and culture collection important in identifying novel species of bacteria?

A: A pure culture is a population of cells or multicellular organisms growing in the absence of other…

Q: What are challenges or difficulties we may encounter for identifying a bacillus cereus microbe ?

A: Bacillus cereus is a gram-positive, facultatively anaerobe, and the rod-shaped bacterium present in…

Q: What is the application of differential centrifugation?

A: In the laboratories, different kinds of techniques and devices are used to separate components of a…

Q: What are the advantages and disadvantages of multitest systems for bacterial identification?

A: A multitest system is the combination of multiple biochemical tests useful in identification for the…

Q: What can researchers learn about a microbial community using metagenomics?

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How…

A: Plasmid training is a way of DNA extraction and purification for plasmid DNA. Many techniques were…

Q: Define about agarose gel electrophoresis ?

A: Electrophoresis is an important tool in biology. It involves separating basic biomolecules: nucleic…

Q: Outline the method of in situ hybridization.

A: In molecular biology, hybridization is defined as a technique in which RNA or single-stranded DNA…

Q: When using a probe to detect an organism, why is it necessary to have some idea as to the organism’s…

A: DNA is the genetic material in all living organisms. Each organism has a unique DNA sequence. Using…

Q: How do microarrays work?

A: A microarray is a laboratory tool used to detect the expression of thousands of genes at the same…

Q: Why are molecular methods especially useful when bacteria are difficult to grow?

A: With the advent of molecular techniques, the culture of unculturable bacteria becomes possible,…

Q: How are molecular probes prepared?

A: Molecular probes are small segments of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which…

Q: How does a dideoxynucleotide differ from a deoxynucleotide? How are dideoxynucleotides important in…

A: Dideoxynucleotides are DNA polymerase chain-elongating inhibitors used in the Sanger technique of…

Q: What are the advantages and disadvantages of differential centrifugation?

A: differential as the name suggests is used to separate heterogenous solutions into their subsequent…

Q: How can a suspect bacterium be fingerprinted?

A: DNA fingerprinting is a technique for detecting a high number of minisatellites in the genome at the…

Q: Where to start with microbial complete genome analysis?

A: Genomic analysis can be described as the process of recognizing, measuring, and comparing the…

Q: When using the blue/ white colony screening method, what do blue colonies represent ?

A: Blue- white screening is used for the identification of recombinant bacteria.

Q: how does growing and identifying an actual bacterium from skin using culture based methods relate to…

A: Staphylococci are gram-positive cocci about 0.5 to 1.0 μm wide. They grow in groups, in pairs and…

Q: Why is  Neurospora a good test organism?

A: Neurospora is a genus of fast-growing orange-red fungus, which lives mainly in tropical and…

Q: What is one situation where Koch’s postulates are impossible to complete? In other words, what…

A: Robert Koch was a scientist who provided four postulates which were designed to prove that there is…

Q: What is a quantitative isolation in bacterial cultivation and how it is done? Explain.

A: To cultivate and count the bacteriophage, plaque formation in solid agar media is used. Plaques are…

Q: How does penicillin enrichment make it easier to isolate mutants?

A: Bacteria are a form of a prokaryote that can be found on the surface of the Earth. Some are harmful…

Q: What type of probes must be used for super-resolutionmicroscopy and why?

A: The super-resolution microscopy involves a series of techniques that allow high-resolution images.…

Q: isolation and purification of bacteriophages from sewage through enrichment centrifugation and the…

A: Isolation of Bacteriophages Centrifuge the Sewage samples at 12000 x g for 30 minutes. It will…

Q: Outline the strategies commonly used to detect for pBR322 in E.coli

A: Plasmids are DNA molecules that exist outside of the chromosomes. They are microscopic, round, and…

Q: What biochemical tests did we use to distinguish staphylococci from streptococci?

A: Staphylococcus and streptococcus both occur in cocci shape and both are gram positive organisms.…

Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?

A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…

Q: What is high throughput screening in the discovery of drug ?

A: Drug discovery is a highly complex and multidisciplinary process which goal is to identify new…

Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…

A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…

Q: Explain gel electrophoresis?

A: DNA is a prerequisite for all life. It contributes to heredity, protein-coding, and the genetic…

Q: E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar…

A: MacConkeys Agar is a selective and differential culture medium. It specifically select Gram negative…

Q: Can gel electrophoresis be a quantitative technique? How so?

A: DNA, proteins, RNA are biomolecules which are very crucial in a cell. Many different techniques are…

Q: What is reductive dechlorination and why is it environmentallyrelevant?

A: Chlorination refers to the process of the addition of chlorine into the water for the disinfecting…

Q: How is the discovery of riboswitches relevant to bacterial pathology?

A: Riboswitches are the gene regulatory mRNA domains that respond to the intracellular concentration of…

Q: What is gel electrophoresis?

A: All living systems are made up of chemical molecules, which interacts with each other to perform…

Q: How metagenomics can provide novelinsight into the microbial world around us ?

A: Microbial communities are analyzed genomically through metagenomics. The process involves…

Q: What is probe? Why it is used in the library screening ?

A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…

Q: How Gel electrophoresis is used ?

A: DNA profiling is the process of determining an individual's unique DNA pattern. DNA profiling can…

Q: What technique is used to stain bacteria? Who developed this technique?

A: Microorganisms are such small living organisms that are less than 0.1 mm, and can be seen only under…

Q: How is bacterial transformation used in biotechnology and How can we tell if we have successful…

A: Bacterial transformation may be referred to as a stable genetic change brought about by the uptake…

Q: Are there technologies that can help make Bacterial Culture and Sensitivity done faster?

A: Microbial culture refers to the culture or growing the bacteria in the laboratories on liquid and…

Genetic analysis

Genetic analysis refers to the process of studying and analyzing changes in chromosomes or genes using different methods in scientific fields such as genetics and molecular biology. It involves the localization and identification of genes and the detection of inherited or genetic disorders caused by mutations or other abnormal variations in our genome.