Q: What is an exconjugant? How do you think that exconjugants were obtained? (It might include genes…
A: Answer: Introduction: Conjugation is the process of transfer of DNA from one bacterium to other by…
Q: What is differential centrifugation? Explain how it can be used to separate cellular organelles.
A: Differential centrifugation : A separation method where the components of a cell are separated on…
Q: Why are the lengths of the cells usually approximated when we record their values for length?
A: Lengths of the cells are usually approximated when we record their values for length as the exact…
Q: What is the significance of temperature control in the polymerase chain reaction?
A: A polymerase the chain reaction (PCR) is a technique for making millions of copies of a single DNA…
Q: Why are knockout mice called that?
A: Introduction There are several ways by which the expression of any gene can be silenced or stopped.…
Q: Why is it wrong to call the created elements cells?
A: Blood is a liquid connective tissue that flows within the blood vessels of the body. Blood is…
Q: Why are some stem cells called pluripotent?
A: The cell is the basic structural and functional unit of life of all living organisms capable of…
Q: In John Gurdon's nuclear-transfer experiments, he used nuclei from tadpole intestinal cells. Do you…
A: Growth alludes to the expansion in mass and size of a body. Development is the cycle where a…
Q: What can happen to chromosomes in cancer cells?
A: Cancer cells vary from normal cells in a range of ways. Normal cells become cancerous after a…
Q: What is the difference between pluripotent and multipotent stem cells
A: Pleuripotent Stem Cells These cells can give rise to all of the cell types that make up the body.…
Q: Were you able to identify a cell undergoing cytokinesis in the onion cell? Why? What extra layer…
A: Cytokinesis is the process of cell division in which the parent cell divides into daughter cells.…
Q: What are long interspersed elements (LINEs) ?
A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…
Q: Why would a cell use SOS repair, considering that it introduces mutations?
A: Answer :- Sos reaction is extreme arrangement when the DNA harm is high that on the off chance that…
Q: How might the technique of affinity chromatography be used to purify lectins ?
A: Lectin is a protein that can recognize carbohydrates and can bind to them. Affinity chromatography…
Q: Once you have transformed these factors into your skin cell, how might you identify your induced…
A: Pluripotent cells are those which have the capability to renew by dividing and it can form three…
Q: Would the application of paclitaxel to a cell make us incapable of performing karyotyping on that…
A: Karyotyoing is the process by which DNA is analyzed . The chromosome structure is usually studied .
Q: What are some of the drugs used to study microtubules?
A: Microtubules are the cytoskeletal structures that are involved in a variety of cellular functions…
Q: difference between cytoplasmic determinants and induction?
A: A process in which cell can influence the fate of development of other cell is known as induction…
Q: What do you think would be the overall effect of reducing the amount of mediator present in a cell?
A: Cells communicate each other and with surroundings for proper growth and functioning. Chemical…
Q: What do you mean by Chromatin Immunoprecipitation?
A: Chromatin immunoprecipitation is an experimental technique used to investigate protein DNA…
Q: What is the role of the enzyme telomerase? In what kind of cell is it normally most active and most…
A: Telomerase is the enzyme responsible for adding telomeres to DNA. The ends of chromosome are called…
Q: What role does chromatin structure play in cell memory and in cell reprogramming?
A: Cellular reprogramming is a process of formation of pluripotent stem cells from mature and…
Q: If the chromatin is removed from a eukaryotic nucleus, what remains?
A: Chromatin is a condensed structure of DNA and proteins that give rise to chromosomes of eukaryotic…
Q: Were you able to identify a cell undergoing cytokinesis in the onion cell? Why? What extra layer…
A: Cell division is defined as the division of the cells for growth, repair and reproduction. Cell…
Q: What is the difference between a cell strain, a cell line, and a clone?
A: All living organisms are made up of highly organized structures known as cells and the cells are…
Q: What would you expect to happen in a cell that had mutations in Mad/Bub proteins that prevented…
A:
Q: What are some of the reasons you might expect of human cells to have more efficient repair systems…
A: DNA (deoxyribonucleic acid) repair refers to the set of processes that are involved in the…
Q: Why does nucleation sites cause the number of microtubules to rise but the length to grow unstable?
A: Microtubules are the structural part of the eukaryotic cells that are formed by the tubulin…
Q: Are all pluripotent stem cells created equal, however?
A: Pluripotent cells are capable of repeated division to form most or all of the cell types but cannot…
Q: In the absence of Delta, why do the cells becomeunsynchronized?
A: In Delta-Notch signaling, Delta from the adjacent cell binds to notch receptor. Intracellular notch…
Q: how is the structure of chromatin loops beneficial to a cell?
A: DNA is the genetic material and in humans it is very large molecule compared to size of cell .
Q: What is denaturation and why is the process harmful to a cell?
A: Introduction A cell is the basic structure of life. A cell is made up of cytoplasm, genetic…
Q: What is chromatin condensation?
A: The sequence of events by which a cell duplicates its genome, synthesis the other constituents of…
Q: Why is it important for a chromosome to be copied before mitosis?
A: Mitosis is a process of cell division in somatic cells. In this cell division, cells divide into two…
Q: What does this figure represent? How do you know if this process is occurring in an animal cell or a…
A: Since meiosis involves two cell divisions, a single starting cell may produce four gametes (eggs or…
Q: What is the function of FAD/FADH2 in cells?
A: Flavine adenine dinucleotide (FAD): FAD is derived from vitamin B12. It is a two-electron oxidizing…
Q: What is the function of remodeling complexes?
A: Nucleosome is the packaging unit in eukaryotes. It is a fundamental subunit of chromatin.…
Q: Does reproductive cloning always involve somatic cell nuclear transfer?
A: Gene cloning refers to the cloning fo genes through various techniques. These copies of genes can be…
Q: what happens to people who get covid-19?
A: Coronavirus disease is caused by SARS CoV-2 virus. The virus affects the respiratory system that…
Q: Why is the spindle assembly check point defective in cancer cells?
A: Introduction: Numerous checkpoints in the cell cycle handles the genomic integrity through very…
Q: How are nucleosomes arranged in condensed 30-nm fibers?
A: The main aim of coiling and various kind of measures undertaken, making a complex structure of DNA…
Q: Were you able to identify a cell undergoing cytokinesis in the onion cell? Why? What extra layer…
A: Red arrow- a cell undergoing cytokinesis in the onion cell.
Q: If a percent difference is a positive number, what does this tell us about the change in weight of…
A: Given, Question has percentage change as positive, we shall assume percentage change as ∆x ie. x…
Q: Why is Neurospora used as genetic material?
A: Neurospora is the genus of Ascomycete fungi and the term "neurospora" indicates "nerve spore".…
Q: What are CDKs? How do they contribute to cell division
A: The Vital proteins involved in the control of cell cycle are Cyclin-dependent kinases / CDKs.
Q: Do you think MinCDE functions in coccoid-shaped cells? Explain your answer
A: The Min System mechanism is based on composition of three proteins viz. MinC, MinD and MinE. This…
Q: You are running general tests in a cell solution and you have detected a large concentration Single…
A: Cell cycle is sequence of events before cell division occurs, it prepares cell for division. Without…
How do you think the end results would be affected if the cells were not treated with a hypotonic solution?
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- You treat some cancer cells with a new therapeutic that you hope will kill them. You run an MTT assay on the results and notice that the sample from the control cells (not treated with drug) turns purple upon addition of the MTT and the sample from the cells treated your drug are clear after adding MTT. As you walk down the hall to run your sample on the spectrophotometer, how are you feeling? (a) frustrated, (b) unsure, (c) excited. I understand that if the cell are alive, they will have functioning mitochondria, the mitochondria will be producing NADH and NADPH which will allow some enzymes in the cell to convert empty T which is colorless, into a colored form as and die. The colorelss means less dye with present, which means the less empty T was converted, meaning that we had less mitochondrial activity, which means we had less live cells. I think that the answer should be (a) frustrated. I am not sure if I am right. Glad if the expert advise.A panel of cell lines was created by human–mouse somatic-cell hybridization. Each line was examined for the presence of human chromosomes and for the production of an enzyme. The following results were obtained:What is differential centrifugation? Explain how it can be used to separate cellular organelles.
- If we dissect the two processes into small steps, can each step be reversible?How did you know that Paclitaxel was inhibiting cell division? What method of observation was used and how did you interpret the images to come to your conclusion that Paclitaxel was inhibiting cell division?Fluorescence-activated cell sorting (FACS) is a powerful technique for separating cells according to their content of particular molecules. For example, a fluorescence-labeled antibody specific for a cell-surface protein can be used to detect cells containing such a molecule. Suppose that you want to isolate cells that possess a receptor enabling them to detect bacterial degradation products. However, you do not yet have an antibody directed against this receptor. Which fluorescence-labeled molecule would you prepare to identify such cells?
- You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions: a) Which technique will you use to isolate mitochondria? b) What happens to the pH of the medium when the medium is kept anaerobic? c) What happens when O2-saturated saline is added to the mixture?Why is it important to model cancer through the generation of induced pluripotent stem cells ? Explain in detail the main findings. Please sort as a list.Would the application of paclitaxel to a cell make us incapable of performing karyotyping on that cell? Why or why not?
- In a cotransduction experiment involving P1, the cotransductionfrequency was 0.53. How far apart are the two genes?What is an electrogenic pump?When preparing cells for karyotyping, in which of the following solutions should the cells be immersed? A.-Isotonic B.-Hypertonic C.-Melted Sugar D.-Hypotonic E.- Saline at human body temperature