How many liters (L) of a 5.0% (m/v) glucose solution would you need to obtain 75 g of glucose?
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- PROBLEM 12.27 (a) Use Table 12.5 to calculate the osmotic pressure of the hemodi- alysis solution at 25 °C. (b) If the osmotic pressure of blood at 25 °C is 7.70 atm, what is the direction of solvent movement across the semipermeable mem brane in dialysis? (Blood to dialysis solution or dialysis solution to blood)You are given as following : 20 µl pure LDH on ice, 2.0ml of 6mM NAD+, 2.0ml of 150mM lactate, and 0.14M CAPS buffer. LDH reaction cocktail has final concentration of 1mM NAD+ and 25mM lactate in 0.14M CAPS buffer. LDH activityis measured by mixing 10 µl of LDH sample and 990 µl LDH reaction cocktail before getting ∆A340/min reading on spectrometer. (a). Describe in detail how you would prepare for your LDH reaction cocktail including how to make dilutions.You are asked by the pharmacist to add 45 mEq of Ca Gluconate in an IV bag of D5W 1000mL. You have a concentrated vial of Ca Gluconate 4.4 mEq/mL, 50 mL. How many mL of this concentrated vial needs to be added to the IV bag?
- A buffer contains 0.015 mol of lactic acid (pK₁ = 3.86) and 0.080 mol of sodium lactate per liter. H₂C OH Lactic acid OH Calculate the pH of the buffer. H₂C. Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of the buffer. O OH Sodium lactate Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of pure water. O Na+ buffer pH: buffer pH change: water pH change: units unitsBSA Volume (1.0mg/ml) (ul) [BSA] (ug/ml) Absorbance 595nm Net Abs 595nm 0.0 0.371 10.0 0.559 20.0 .707 25.0 .902 Please help me calculate BSA and Net Abs (explaination and details pls)A 60 kg patient is on a continuous IV constant rate infusion of theophylline at 30 mg/h. His steady-state concentration is determined to be 12 mg/L. If you assume the theophylline distribution volume to be 22 L, what would be the patients plasma concentration 4 hours after the continuous infusion stopped? Please select one: 0 a. 6.5 mg/L b. 7.6 mg/L c. 4.8 mg/L d. 8.4 mg/L e. 3.7 mg/L
- A KCC student titrated 100.00 mL of a NaOH solution using 55.00 mL of a 0.1000M H2SO4 solution. How many moles of acid were used ? This is part 1 of a titration problem H2SO4 + 2 NAOH Na2SO4 + 2 H20 O A. 0.0055 mol B. 0.0100 mol O C. 0.100 mol D. 0.550 mol O E. 5.50 molYou record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equationData for standard glucose concentrations Glucose concentration (mm) 0 0.5 1 1.5 -~~ 2 2.5 A Data for plasma samples B Patient Timepoint (hours) C D E 0 0.5 1 2 0 0.5 1 2 0 0.5 1 2 1. Draw a standard curve of absorbance at 420nm against glucose concentration in mmoles/L. We recommend you use Excel or a similar programme to insert the graph Alternatively, you can draw by hand on graph paper and insert a photo of the graph. OON 0 0.5 1 Absorbance 1 0.000 0.477 0.947 1.234 1.733 2.160 2 0 0.5 1 Absorbance Absorbance 2 0.000 0.482 0.941 1.236 1.721 2.276 Absorbance Glucose concentration Glucose concentration Abs 1 Abs 2 Average from graph (mM) in plasma (mm) 0.635 0.632 0.629 1.368 1.370 1.369 1.382 1.388 1.385 1.151 1.147 1.149 0.566 0.844 0.869 0.772 0.395 0.397 0.399 0.850 0.858 0.854 0.738 0.744 0.741 0.539 0.531 0.535 0.776 0.780 0.778 1.475 1.481 1.478 1.446 1.444 1.445 1.302 1.296 1.299 0.560 0.563 0.848 0.846 0.871 0.870 0.774 0.773 Average 0.000 0.480 0.944 1.235 1.727 2.218 0.440…
- BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.What is the rate in mL/H if a 110lb-patient is on Norepinephrine (1mg/mL) 16mg in NSS 100mL running at 0.85mcg/kg/min? choices23.4 ml/H17.8 ml/h18.5 ml/H19.2 mL/HYou obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.262 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).