In order to determine the purity of a DNA sample. spectrophotometry can be carried out at _______ to measure DNA, and _______  to measure protein. The ratio _______  is then calculated, and a number between _______  indicates higher purity.

Q: ) *When double-stranded DNA is heated, the two strands separate into single strands in a process…

A: Factors affecting Tm The temperature at which half of the dsDNA is denatured is called T_{m}…

Q: In figure 10.16 , how would the curve appear if the GC content of the DNA sample were increased? How…

A: Introduction: DNA is made of four nucleotide bases that are adenine (A), thymine (T), guanine (G),…

Q: A plot showing the % of denaturation as a function of temperature for a melting point of a DNA…

A: DNA is a double stranded helically wound macromolecule. Each strand is made up of a sequence of…

Q: Describe the characteristics of the extracted DNA, such as colour, shape, size, and consistency.

A: DNA is the genetic material of most organisms and it is more stable than RNA.

Q: DNA molecules of different sizes are often separated with the use of a technique called…

A: Electrophoresis is a technique that is used to separate molecules based on their sizes.

Q: Suppose there are three tubes containing following samples in the laboratory. The three tubes are…

A: DNA(deoxyribonucleic acid) is defined as the building block of life because it carries all the…

Q: In a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the…

A: Asked : Reason of Absorbance increases by disruption of DNA structure.

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: DNA (Deoxyribonucleic acid) and RNA are two examples of nucleic acids (Ribonucleic acid).…

Q: a) Explain the effect of the guanine:cytosine ratio on melting temperature of DNA. b) The…

A: (a) Guanine : cytosine ratio is the ratio of base pairs in the DNA. There are four bases in DNA -…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: Given values:  Total DNA extracted = 500 μL Total weight of wheat used for DNA extraction = 200 mg…

Q: The illumina method of sequencing uses a unique type of nucleotide building block. What is the…

A: The illumina sequencing is a DNA sequencing method used to determine single nucleotide bases of a…

Q: DNA is an attractive option for long-term data storage because hydrogen bonding between single…

A: Need to find whether the DNA is a good option to store data or not.

Q: TRUE OR FALSE a) The long solenoid structure of the chromatin material binds to a protein scaffold…

A: The DNA of a cell and its related proteins make up chromatin. In eukaryotic chromatin, histone…

Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer? Table salt dissociates…

A: DNA is a double-helical structure whose backbone is made up of sugar-phosphate and bases are present…

Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…

A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…

Q: The electrical current created during gel electrophoresis forces DNA to move from the negative end…

A: Answer.  DNA or Deoxyribonucleic acid is made up of nucleotides. A nucleotide contains a phosphate…

Q: DNA strands that can form a double helix are said to be Select an answer and submit. For keyboard…

A: Deoxyribose Nucleic Acid or DNA is a double-stranded molecule with the shape of a twisted ladder.

Q: suppose there are three tubes containing DNA RNA and protein samples in THE laboratory. the three…

A: Introduction: Those biomolecules that require in large amount to keep the body fit and healthy are…

Q: Put the following pieces of DNA in order that they would appear reading from the top (nearest the…

A: First of all lets convert all of them into single unit 1kb = 1000bp So, 21000bp =21kb 10000bp = 10kb…

Q: Four nucleic-acid samples are analyzed to determine the percentages of nucleotides they contain.…

A: DNA and RNA are nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid whereas…

Q: Evidence or approaches that led to the discovery of the DNAdouble helix includea. the determination…

A: Introduction: DNA is the hereditary material present in the nucleus that is responsible to transfer…

Q: Fill in the blanks: In electrophoresis, an electric current is applied to the matrix of a gel-like…

A: Gel electrophoresis is a technique that is utenly used in molecular biology laboratories. It…

Q: Quantification of DNA can be done by using a Nanodrop, a UV spectrophotometer, by measuring its…

A: The formula given for determining the dsDNA concentration is give as: dsDNA concentration= 50 μg/ml…

Q: During the process of Gel Electrophoresis, DNA will move through the gel towards the-------end of…

A: DNA is the genetic material. It is responsible for regulating cell activities.

Q: A linear piece of DNA is cut as follows: cut with Ecorl - get 1kb and 4 kb band Cut with Bamhl - get…

A: Ans- According to question For linear DNA – Upon Cutting by for Eco R1 it formed – 1kb and 4kb   =…

Q: A student was analyzing the base composition of a DNA sample, but has lost all the data except the…

A: DNA nucleotide base structure: Each individual unit of the DNA nucleotides is made up of a…

Q: Which of the following items do not affect the melting temperature of DNA? A length of the DNA B the…

A: Melting temperature ( Tm): half of DNA gets denatured (melts). Tm = 2* (A+T) + 4* (G+C)

Q: What is a DNA Ladder? a solution added to a DNA sample to give it color for an electron microscope.…

A: DNA:  The hereditary substance in humans and almost all other animals is DNA, or deoxyribonucleic…

Q: Which of the following is NOT true of deoxyribonucleic acid (DNA)?   A. None of the other four…

A: DNA - deoxyribonucleic acid is a polymer of nucleotides which acts as the genetic material in all…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: Given wheat 200 milligram germ extraction volume. = 500 microlitre Sample used for analysis=…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: DNA generally stands for Deoxyribonucleic acid It is generally double standard Purine and…

Q: Blue prints of body design are stored in the DNA. Why?

A: Genetic material is the medium through which genetic information passes from a parent cell to its…

Q: he figure shows that the average distance between base pairs measured parallel to the axis of a DNA…

A: The nucleic acid polymer has nucleotide as its monomeric unit. synthesis of nucleotides is an…

Q: The sum of the lengths of DNA fragments 1-3 equal the total original length?  True   /   False

A: Answer : The sum of the lengths of DNA fragments 1-3 equal the total original length :    false.

Q: Describe the denaturation of DNA including the factors that can affect it and the kind of forces…

A: Denaturation is the process of loss of the native structure by applying some external denaturing…

Q: Why evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?

A: The characteristics of an evenly spaced sequence are:-  Evenly-spaced peak, each being…

Q: Place the following stages of a physical mapping study in theirmost logical order:A. Clone large…

A: Artificial chromosome- laboratory constructed cloning vectors that carry larger DNA insert than…

Q: proteins are denatured and eliminated during the DNA extraction process. how can we determine the…

A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…

Q: If you eat DNA all the time, why aren’t you harmed by it? What implication does this have on the…

A: DNA or deoxyribonucleic acid is the genetic material found in all cells. It carries the coded…

Q: The 260/280 ratio of pure DNA is between 1.8- 35 2.0. The absorbance of DNA at different 30…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: You are given a tube containing 1.5 ml of a DNA solution. The concentration of the DNA solution is…

A: The nucleus of a cell contains the nucleic acids which are responsible for the passing down of…

Q: The technique of gel electrophoresis gives scientists an effective way to see some differences…

A: DNA stands for deoxyribonucleic acid. It is a double helix made up of two polynucleotide chains that…

Q: The Evidence shows which suspect was at the crime scene? ( image of murder investigation) A)…

A: DNA bands can appear thick and thin in the agarose gel electrophoresis, thicker and darker DNA bands…

Q: The concentration of digested and cleaned DNA is found to be 5μg/mL. How much of your DNA sample (in…

A: Answer: DNA = (Deoxyribonucleic Acid) It is a double stranded , constitutes as the genetic material…

Q: Which of the following statements is TRUE about buffers in the separation of DNA strands via gel…

A: Agarose gel electrophoresis is a fundamental technique in molecular biology that is used to view and…

Q: hy is there a difference between how the DNA looks between a whole food sample (strawberry or peas)…

A: Genes can be found in any food, whether it comes from plants or animals. The majority of the DNA in…

Q: A sample of DNA is collected from an organism. It is analyzed and determined that 20% of the DNA is…

A: DNA is made up of long chain of polynucleotides. Each nucleotide is a monomer and is comprised of…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.