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In the traditional alkaline lysis method there is Solution I, II, and III, what is the purpose of NaOH in solution II?
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- 1. Calcium in a sample solution is determined by atomic absorption spectroscopy (AAS). A stock solution of calcium is prepared by dissolving 1.834 g CaCl, 2H,0 in water and diluting to 1 litre. From this stock solution, the second stock solution is prepared by the dilution factor of 10. Three standard solutions of calcium are prepared from the second stock solution with the following dilution factor: 20 (first standard solution), 10 (second standard solution) and 5 (third standard solution). A blank solution is prepared as well. Absorbance signals of AAS are as follows: 1.5 (blank solution), 10.6 (first standard solution), 20.1 (second standard solution), 38.5 (third standard solution), 29.6 (sample solution). (Molar mass; Ca = 40.00 g mol-, CI = 35.45 g mol-, 0 = 16.00 g mol-', H = 1.00 g mol-1) a. Construct ONE (1) plot that could be used to represent the calibration curve. b. From the plot of Q1a, determine the amount of calcium in parts per million.A sample of material contains the components NaOH, Na2CO3, NaHCO3 , or possible mixtures of these. Two samples, each weighing 1 .000 gram, are dissolved in water. To one sample phenolphthalein is added and the solution is titrated cold with 1.038 N acid, of which 17.96 ml are required. The other sample is titrated cold with methyl orange as an indicator, and 21.17 ml of the same acid is required. Calculate the percentage of alkalies present.For a 50ml beaker, 0.4532g of pure iron III oxide was weighed, to which 1 ml of a concentrated hydrochloric acid solution was added and then 20 ml of deionized water was added to the beaker. After mixing, the resulting solution was transferred to a 50.00 ml flask and the volume was made up with deionized water. Then 15ml of this solution into a second beaker to which 10ml of an iron iii chloride solution of 0.200 M concentration was added. Calculate the final concentration of the iron iii cation solution expressed in mol/L and in g/L, considering that the volumes are additive.
- Explain why a dialysis solution must have a low sodiumion concentration if it is designed to remove excesssodium ions from the blood.3.) In the assay of NaHCO3, 3.0g of the solid is dissolved in 25mL water. What is the normality? How many mL of 1N H2SO4 will be required to neutralize this solution? From this volume of acid, compute the percent purity of NaHCO3.Pretend that you are conducting this experiment in the laboratory and answer the following questions. You are asked to weigh 2 g of lithium and add it to 1500 ml of distilled water +2 drops of indicator, knowing that the density of water is 1 at room temperature while conducting this experiment. After knowing the amount of LiOH, you need to dilute to prepare 0.025 M of LiOH in a 3000 ml(v) solution. Show your calculation and units.Knowing that the initial volume that you used was 1500 ml(v). Use this equation to calculate ( CLiOH x VLiOH) before dilution = (CLiOH x VLiOH) after dilution
- Write the balanced formula unit equation for the complete neutralization of dilute H3PO4 with CaOH)2 in aqueous solution. What is the sum of the coeffioenes? Do not forget coefficients of one) O 10 012 OH 05 0.18What is the purpose of a “salt bridge?1. A sample of pure sodium oxalate, Na2C2O4 , weighing 0.2856 g is dissolved in water, sulfuric acid is added, and the solution titrated at 70°C , requiring 45.12 mL of a KMNO4 solution. The end point is overrun and back-titration is carried out with 1.74 mL of a 0.1032 N solution of oxalic acid. Calculate the normality of the KMNO4 solution. Hint: A redox reaction is involed between oxalate and permanganate. Look at the changes in oxidation state by determining the balanced redox reaction: 5C20,2 + + 2MN2+ 2 Mn04 + 10H* → 10CO2 + 8H20
- You have 10 mL of an 8% aqueous solution of H2Cl6Pt. (a) Calculate the platinum in this solution as wellas (b) the molar concentration of the platinum in this solution. The density of the solution is 1.05 kg.L-1.a. Calculate the % Pt in the solution: b. Calculate the molar concentrationof the platinum in the solution. [) Describe the preparation of 1.00 L of 0.150 M KMNO: from a solid KMNO4 1. reagent. 2. . * A sodium hydroxide solution is standardized by titrating 0.8592 g of ordinary standard potassium hydrogen phthalate (204.22 g/mol) to a phenolphthalein end point, requiring 32.67 ml. What is the molarity of the NaOH solution? co,K coK + NaOH + H0 CO,H Pnim ydog phala(c) What is a standard solution? What piece of glassware is used to prepare a standard solution and describe how a standard solution of sodium carbonate may be prepared in the laboratory?