Ligation is an essential step in the cloning process. It refers to the joining of the gene of interest to the vector using DNA ligase. Determine FOUR (4) control groups which are important to determine the success of this step and also to troubleshoot if any problem should occur. (i) (ii) In an experiment, 100 ng vector was added into ligation reaction with 50 ng of insert (500 bp). The desired vector: insert ratio of 1: 3 was used. Determine the size of the vector.
Ligation is an essential step in the cloning process. It refers to the joining of the gene of interest to the vector using DNA ligase. Determine FOUR (4) control groups which are important to determine the success of this step and also to troubleshoot if any problem should occur. (i) (ii) In an experiment, 100 ng vector was added into ligation reaction with 50 ng of insert (500 bp). The desired vector: insert ratio of 1: 3 was used. Determine the size of the vector.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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