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- Consider the solutions shown in the containers below. The composition of each solution is given in the image. 0.15 Mlactose isotonic hypotonic hypertonic crenation hemolysis no change A 0.28 M Urea B Fill the blank with the appropriate term from the list below. 0.16 MKCI The tonicity of the solution in container C isYou are growing yeast cells in your lab. The Yeast Growth Medium contains 56 mM glucose (Mol. Weight 180 g/mol) and 20 mM HEPES (MW 238 g/mol). The yeasts only grow if the medium is at the exact pH=6.8. You have HEPES and glucose powder, water, hydrochloric acid (HCl) and Sodium hydroxide (NaOH), all the necessary equipment to prepare the 100 ml that you need. Calculate and explain how you make the Yeast Growth Medium:Give clear detailed Solution
- Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…A biochemist carefully measures the molarity of salt in 56. mL of photobacterium cell growth medium to be 82. mM. Unfortunately, a careless graduate student forgets to cover the container of growth medium and a substantial amount of the solvent evaporates. The volume of the cell growth medium falls to 3.1 mL. Calculate the new molarity of salt in the photobacterium cell growth medium. Be sure your answer has the correct number of significant digits. | mMSubstrate Concentration 2 ts H2O2 1 ts H2O2 1 tbs H2O2 2 tbs H2O2 ½ ts Yeast Solution 2 tbs Yeast Solution Enzyme Concentration 1 ts Yeast Solution 1 tbs Solution Yeast Temperature 2 tbs Ice Water 1 tbs Ice Water Room 1 ts Hot Water 2 tbs Hot Water Temperature pH Acid Distilled Water Base | 2 Base 2 tbs Solution Acid ts 1 ts tbs Solution Solution Solution Multiple Choice 16. What should be observed in the experiment? a. Amount of bubbles/ effervescence released c. Length of effervescence formation b. Time when test substance was added until a reaction occurs d. All of the choices 17. What does the effervescence represent? a. Carbon dioxide was released b. A reaction took place b. Enzymes are limited by many factors 18. What amount of substrate concentration produced the greatest amount of effervescence? a. ½ ts H2O2 19. Which of the following had the longest time for effervescence to stop forming? a. ½ ts H2O2 20. The solution that showed the shortest time of forming bubbles is: a. ½…
- McConkey Agar has been requested by a hospital that is investigating cases of suspected Salmonella food poisoning. The formula on the bottle of powdered media is 50 g / 1000 mL deionized water. How many grams of media would be required to prepare 90 plates assuming 20 mL per plate? O 150 O 75 90 O 180The authors of this paper also tested another minimal media (Media C) which is exactly the same as Media B except that the pH is 7.4 instead of 7.1. Which media components would be different in Media C? For each component that is different, state if will be larger or smaller than the amount in Media B.Give Explanation Detailed Solution of sub parts..(no need handwritten answer)
- Dextrose 5% in water solution is an example of hypertonic solution. True False?15. Calculate and explain how to make 800 mL of a 5% solution from a 20% solution. 16. Calculate and explain how to make 1500 mL of a 1:9 dilution from a 12M H3PO4 solution. 17. Calculate and explain how to make 800 mL of a 1/20 dilution from a 4M H3PO4 solution.Please show the solution. 2.