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- asapp plssss dsadwafasfcas1. Penicillin is hydrolyzed and thereby rendered inactive penicillinase, an enzyme present in some penicillin-resistant bacteria. The molecular weight of this enzyme in Staphylococcus aureus is 29.6 kilo Daltons (29.6 kg/mole or 29,600 g/mole or 29,600 ng/nmole) The amount of penicillin hydrolyzed in 2 minute in a 10-mL solution containing 109 g (1 ng) of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change appreciably during the assay. [Hint: Convert everything to the same concentration terms] Show all calculations and include spreadsheets and graphs to determine Km, Vmax and kcat for this enzyme. Make sure your final answers have correct units. [Another hint: Note that [S] and amount hydrolyzed are already in concentration terms. So you don't need to worry about the volume for calculating [S] and V. ] Penicillin concentration (microM) Amount hydrolyzed (nanoM) 1 110 3 250 5 340 10 450 30 580…CH3 Br2 [0] A -H2O NH3 NaOH -NaBr FeBr3 D Cu,180°C CH3 Br2 Mg CH3CH-O -B H,O [0]. C -D- E FeBr3 efir efir H* 8. CH;CHCH2CH2OH FS04 #so) -1)03 2)H0 -H;O2 H30 Al-O3 CH3 CH;MgJ CH5J H,O [H, Hg 2КОН 9. Br2 CH;CH=CH, spirt meh.
- H30* d. [1] CH3OH, H* CH30 HO. MgBr е. [2] (2 equiv) [3] H20 HO [1] SOCI, f. [2] CH3CH,CH,CH,NH2 [3] LIAIH4 [4] H2OA. B. H₂C C. EtO. Bz Ph -CI CH3 OEt AICI 3 NaOCH3 CH₂OH, A 1) NaOEt 2) Br2, CH3COOH 1) NaBH4 2) H3O+ workup H3O+, AAaBbCCL I Normal T No Spac. Heading 1 Heading 2 A . A- Styles Paragraph Alb ol o2 B Nephrotic Syndrome 1.3 A 60-year-old woman with the history of rheumatoid arthritis, smoking 60 packs/y, chronic obstructive pulmonary disease and treated hypertension underwent a screening colonoscopy and the felt poorly for a week. She saw a physician, who referred to the local clinic where they did a list of blood tests as indicated below: 業 Tests Results Ref ranges 3.7 mg/di 35mg/dl | 12.5 ml/min 148mg/dl 19mg/di Creatinine Females <1.2 BUN 5-20 Creatinine Clegrance 75-115 C3 80-200 C4 15-80 4.1 g/di positive Albumin 3.5-5 Cryoglobulin screen Serum protein electrophoresis (SPE)- polyclonal hyperalobinemia with no monoclonalimmunoglobulin spike identified. Urine sediment: no RBC, 4 8WBC's/hpt, granular and hyaline casts 1.3.1 What disease state is the most likely explanation for the patients results. Nephrocalcinosis 1.3.2 What is the clinical signiticance of polyclonal hypergammaglobunemia with no…
- AH = - 1.2 kcal/mol; Kp = 16 nM OH 1 HO AH = - 6.0 kcal/mol; N. Kp = = 76 nM OH 2 HOl. AH = - 5.5 kcal/mol; Kp = 0.5 nM OH (iii) By considering the enthalpies given, what do you think this suggests about the target (e.g. the binding pocket of an enzyme) that the molecule binds to?HhhbbvccHELPPP NOWWW PLEASE