Please identify the amino acid shown below: Arginine ΝΗ H₂N m -1 NH₂ OH If this amino acid was at pH=11.0, what would be its overall charge? (Hint= the pKA for the its R-group is 10) Arginine -0.500 X
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- In the figures are the titration curves of two amino acids. Consider the first titration. 00 000 pH 10.0 4 14.0 10 9 12.0 3 نیا 8.0 Select the pK, values of each functional group observed on the titration curve. 2 9 6.0 40 20 0.0 0.5 1.0 1.5 2.0 2.5 3.0 equivalents of [CH] Identify which amino acid is being titrated. arginine asparagine histidine lysinePlace each charge form of alanine under the pH condition where it would be the predominant form. The pK₁ values for the carboxyl group and amino group of alanine are approximately 2.3 and 9.7, respectively. pH 11 H | H₂C-C-COO NH₂ Does not occur in significant amounts at any pHAssume a pH of 7.0 in aqueous solution. Please draw a tripeptide containing (in this order) a residue with a residue with an non-polar sidechain containing more than five carbons, a positively (+1) charged sidechain, and a residue with a polar (but not charged) sidechain with more than two carbons. Please give the names of the amino acid residues that you drew.
- Amino acid structure For any ionizable group, indicate the pka value and draw the structure that would be expected at the pH inside the cell (~7.4).A peptide has the sequence Glu-His–Trp–Ser-Gly–Leu-Arg-Pro–Gly The pK, values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 4.25 histidine 9.17 1.82 6.00 tryptophan 9.39 2.38 serine 9.15 2.21 glycine 9.60 2.34 leucine 9.60 2.36 arginine 9.04 2.17 12.48 proline 10.96 1.99 Calculate the net charge of the molecule at pH 3. net charge at pH 3:A mixture of Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74), Phenylalanine (pl 5.48) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2. v First 1. Aspartic Acid v Second 2. Histidine v Third 3. Lysine 4. Phenylalanine v Fourth 5. Threonine v Fifth 6. No separation
- a) Draw amino acid (L- Cysteine) and determine the net chagre at each pH (1-3-7-10-13-) b) Calculate the pl for amino acid (Cysteine) 3. This question requires you to use an amino acid with an ionizable R group. Use the Amino Acids with lonizable R Groups chart to determine which amino acid you will be using. For student number 000123456; 2-6 and using the chart for Question 3 this corresponds to Glutamic Acid. Amino Acid 3=Z= pH 1 Draw the structure of L-amino acid 3 at the pH's in the table below and determine the net charge at each pH and the gl for amino acid 3. 10 Name of amino acid 3: 13 Cysteine Structure Net ChargeAmino acid Cys has ionizable groups with pKa values 2.0, 8.0, and 10.0. Determine the charge on the Cys at the following pH. Be sure to write the charge in fraction with sign in front (for example, +1/2, -1/5, and +7/8). Charge when pH is at 1.0? _________ Charge when pH is at 9.0? _________ Charge when pH is at 8.0? _________ Charge when pH is at 10.0? _________Given the following peptide, answer following questions 1-5: CO CH2 O || CH2 O H3N-CH-C-NH- CH-c-OCH3 | 1. Is this a dipeptide or tripeptide? 2. How many amino acid components/residues are there? 3. Name these amino acids. 4. What kind of bond links these amino acids? 5. The above structure represents the major ingredient in some commercial product(s). Identify the name of this major ingredient or the commercial product(s). Given that the hemagglutininin protein in influenza virus contains a remarkably long a-helix with 53 residues, answer questions 6-7. 6. How long is this a-helix (in nm)? Show all calculations. 7. How many turns does this helix have? Show all calculations.
- A peptide has the sequence Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly The pK₁ values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 4.25 histidine 9.17 1.82 6.00 9.39 2.38 9.15 2.21 9.60 2.34 9.60 2.36 9.04 2.17 10.96 1.99 tryptophan serine glycine leucine arginine proline Calculate the net charge of the molecule at pH 3. Calculate the net charge of the molecule at pH 8. 12.48 net charge at pH 3: net charge at pH 8:Determine the net charge (to the nearest integer) on the following peptide at pH 5 AND pH 12. Estimate the isoelectric point (pl) for this peptide: H2N-Leu-Gly-Lys-Glu-COOH Assume the pK,s for functional groups are: alpha-amino 6. alpha-carboxy sidechain-amino (Lys) 10.5 sidechain-carboxy (Glu) 4.2 O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =6.6 O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =3.1 O Net charge at plH 5 and 12 is +2 and -1, respectively, pl =7.5 O Nct charge at pH 5 and 12 is +1 and -1. respectively,. pl -4.5 2.Draw the Ala-Ser-Leu-Asp polypeptide (perspective formula) showing the fully extended backbone geometry (all angles +/- 180°). Include correct stereochemistry for L-amino acids. Label all bond rotations representing the angles at each residue