Problem 4. Calculate the amount of rice bran (12.5% CP), com (8.5% CP), soybean meal (49% CP) and copra meal (20% CP) in formulating 100 kg diet with 20% CP. Assume that the energy mixture will have a ratio of 2:1 and protein ingredients proportion is 1:3. Show your solution:
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- PROBLEM 2: A biochemist dliscovers and purifies a new enzyme, generating a purification table below The step of protein purification Total Total Specific Total The multi- Total activity yicld (%) cnzyme fold protcin (mg) maltifold activity increase in purification (units) purity Whole-cell lysate 8.4 LO 500 l00 4200 40% ammonium 22.2 95 2.64 9.64 180 3990 sulfate Ion exchange 90.2 251 6.63 68 3787 55.7 Gel filtration 50 5000 to0 119 1.8 11.9 (a) From the information given in the table, calculate the specific activity of the enzyme, total yield (%), multi-fold increase in purity, and total multifold purification after each purification procedure. b) Which of the purification procedures used for the enzyme is most effective (Le., gives the greatest relative increase in purity)? (c) Which step - ion-exchange or gel filtration chromatography - causes the greatest loss of the protein. (d) If you wanted to use only one purification step, which technique would you choose?ent Page Form Combine Files Protect Flatten File Ch. 7] PROBLEMS Share 390% Enzymes-2.pdf Help + < Problems 125 (142/434) Capture the ronction: Add Bookmark 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol 1¹¹): 5.0 Initial velocity (umol l´¹ min¯¹): 147 Calculate Km and Vmax 6.67 10.0 20.0 182 233 323 125 40.0 400Problem # 3: Calculate the number of moles of ATP produced from the complete oxidation of 900 g glucose in the cells. Show your computation/solution.
- Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…*finish the equation problem* my patients order is written in apothecaries unit dose. The package says aspirin 325 mg. I need to figure out how many tablets I need to administer In apothecaries It indicates that its 10 grains of aspirin I know 1 grain is equivalent to approximately 65 mg so all I have to do is solve the problem 65mg 1 grain set up the second fraction with the unknown quantity in the approximate position X1 grain put the fractions into a proportion 65 mg X 1 grain = 10 grains cross multiply 65mg * 10 grains = X *-1 grain Solve for X by dividing sides of the equation by 1 grain and canceling unitsv (mm/sec) 5.0 4.0 3.0 2.0 1.0 0.0 0.0 1.0 0 Estimate from the M-M plot below: Vmax, Km, Keat, and enzyme efficiency 2.0 [ET] =2.0 mM 3.0 [S](MM) SmartArt 4.0 5.0 6.0 7.0 Box
- Problem 1: Calculate the hydrolysis of ATP at pH 7 at 37°C under steady state conditions in which the concentration of ATP, ADP and Pi are maintained at 10-³M, 10 M and 10-2M, respectively. The AG' of hydrolysis of ATP at pH 7 = -32,217 J/mole, R = 8.314 JK ¹mol-¹, T= 273 + 37 K. ATP ADP + Pi(problem 2.25 textbook, see page 41) Stoichiometry and Incomplete Reactions For production of penicillin (C16H18O4N2S) using Penicillium mold, glucose (C6H12O6) is used as a substrate and phenylacetic acid (C8H8O2) is added as a precursor. The stoichiometry for overall synthesis is: 1.67 C6H12O6 + 2NH3 + 0.5O2+ H2SO4 + C8H8O2 à C16H18O4N2S + 2CO2 + 9H2O What is the maximum theoretical yield of penicillin from glucose in g/g? When results from a particular penicillin fermentation were analyzed, it was found that 24% of the glucose had been used for growth, 70% for cell maintenance activities (such as membrane transport and macromolecule turnover) and only 6% for penicillin synthesis. Calculate the yield of penicillin from glucose in g/g under these conditions. Batch fermentation under the conditions described in part b) is carried out in a 100-liter Remember that only 6% of the glucose is used for penicillin synthesis. Initially, the tank is filled with nutrient medium…Problem 3 Is the protonation state of the tripeptide, Glu-Lys-Cys, as drawn below feasible? If so, at what pH? If not, why not? What is the isoelectric point for this tripeptide? O OH SH *H,N NH
- Activity 1: Practice Problems 1. In humans, phenylketonuria (PKU) is a disease caused by an enzyme deficiency at step A in the following simplified reaction sequence, and alkaptonuria (AKU) is due to an enzyme deficiency in one of the steps summarized as step B: A B phenylalanine tyrosine CO₂ + H₂O A person with PKU marries a person with AKU. What phenotypes do you expect for their children? wildtype O PKU O AKU O Both PKU and AKUStudy problem 2 4. A coworker has just isolated a copper enzyme that catalyzes the conversion of oil sludge into soluble alcohols in the presence of O₂. There are two Cu atoms per protein, which consists of a single polypeptide chain. As the bioinorganic chemist on the project, you are given unlimited quantities of the protein for the purpose of determining the active site structure. You have at your disposal a number of physical techniques, including NMR and EPR spectrometers, a magnetic susceptometer, a Mössbauer instrument, an X-ray absorption beam line, a UV-VIS spectrophotometer, a Raman spectrometer, a magnetic circular dichroism instrument, but, alas, no X-ray diffractometer. You have time to complete measurements by only three techniques before you have to give a report to your colleague. Describe what measurements you would make, in what order you would make them to get the most out of time, what results you might expect, and how you would use this information to characterize…Question: Determine the Km and Vmax for this enzyme/substrate combination. [Substrate] (mM) V0 (mM/min) 0.25 0.183 0.50 0.356 1.00 0.665 2.50 1.45 5.00 2.35 What is the concentration of substrate necessary to achieve a turnover rate of 1.00 mM/min?