PROTEINS 1. What will happen to free tyrosine after being subjected to xanthoproteic analysis (is it positive or negative, include color) 2. What will happen to lysine after being subjected to millon's analysis (is it positive or negative, include color)
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PROTEINS
1. What will happen to free tyrosine after being subjected to xanthoproteic analysis (is it positive or negative, include color)
2. What will happen to lysine after being subjected to millon's analysis (is it positive or negative, include color)
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- hi, can I please get help on a case study on nueroanatomy I have been struggling for a couple of hours now and can't seem to understand the study to answer the following questions. is there any way or format that i can get help. I would really appreciate it. thanks! 1. Based on the information in the case, what is the most likely neuroanatomic location for a single lesion that can explain all of the patient’s symptoms and signs? In your own words, explain how you arrived at that localization. 2.What are some possibilities for the nature of the lesion (e.g., stroke, tumor, trauma, etc.)? In your own words, explain your rationale for these options. 3. How does the laboratory data and neuroimaging demonstrate the actual lesion for the patient? Describe how you interpret the data in your own words. 4.How was the patient was treated, and how did they subsequently fare? Describe the treatment plan in your own words.PLEASE ANSWER BRIEFLY. Thank you. 5. In hairy cell leukemias (HCL), tartrate resistant acid phosphatase is demonstrated. In your opinion, is this the most reliable test to diagnose HCL at present. Explain in not more than 3 sentences. 6. The use of monoclonal antibodies to detect cluster of differentiation (CD) in leukemic cells, mostly immature cells is common in the laboratory. Why do you think this is more valuable than morphological examination of cells in the blood/bone marrow cells.b. Cleavage with chymotrypsin produces the following fragments: Band A: CN , NLQY, GIVEQCCHKRSEY Band B: F, Y, DPTKM, IACCVRGF, RTTGHLCGKDLVNALY Cleavage with Staphilococcus aureus V8 protease produces the following fragments: Band A: GIVE, YNLQNYCN, QCCHKRCSE Band B: PTKM, RTTGHLCGKD, LVNALYIACGVRGFFYD What is the amino acid sequence of the protein? Type your response
- fill in the following.6. CASE STUDY. Your patient is a 2-week old child with elevated levels of phenylpyruvic acid, phenyllactic acid, and phenylacetic acid in his urine. He has elevated levels of phenylalanine in his blood. You do a liver biopsy and prepare a cell-free extract from the liver cells. To this you add tetrahydrobiopterin and radioactive phenylalanine and find that the production of radioactive tyrosine is only 1% of that which you would expect in the case of a normal person. (a) Name the defective enzyme (you will have to do some detective work). (b) Write out the reaction catalyzed by the defective enzyme (use structural formulas). What is the stoichiometry? (c) Assume that you have identified the defective enzyme. Assume that you have a polyclonal antibody to that enzyme from normal persons. Using the antibody, you find no evidence that the enzyme is expressed in liver cells of your patient; in a liver cell from a normal person, however, the antibody would show that the enzyme was expressed.…True or false 1. Heterogenous antibodies used for structural studies can be obtained by monoclonal hybridoma technique. 2. PrP(Sc) is largely alpha-helical structure.
- help. 1. To produce a specialized-transducing mycobacteriophage construct, a cosmid bearing the allelic exchange substrate is electroporated in Mycobacterium smegmatis, followed by an incubation at 30°C. True/ False 2. A recombineering system involves the over-expression of recombinase proteins, which allows the use of a short DNA fragment as allelic exchange substrate, but can also be combined with the use of a mycobacteriophage construct. True/ False6_c. In cancerous cells, CpG islands are: where intercalating agents are found demethylated methylated.A. Prepare a diagram with labels showing the similarities and differences among the four major types of ELISA based on their capture and detection systems. A1. Give at least one clinical application for each type of ELISA. B. What is the purpose of adding horseradish peroxidase, alkaline phosphatase, and their respective substrates?
- Which letter represents the target site of furosemide (Lasix)? E- -BThis is homework not a test! From NTSA case study https://static.nsta.org/case_study_docs/case_studies/cystic_fibrosis.pdf Please help with questions 2, 3 and 4 of part four 2. "The successful use of gene therapy to cure SCID syndrome (2000) is hoped to be a permanent cure for those patients because a good copy of the problem gene was inserted into the patients' blood stem cells in the bone marrow (hematopoietic stem cells). Once white blood cells enter the blood stream they have a limited life span, on the order of a few week to months. The blood stem cells are the cells that create more white blood cells to replace those that are lost. If the gene was only inserted into the circulating mature white blood cells, the patient would only be temporarily cured until those cells were used up or died." The current gene therapy approaches to cure CF involve inserting a functional CFTR gene into the mature epithelial cells of the lungs. In light of the preceding paragraph, do you think that…You have the following reagents available Rat anti-Keratin 8 antibody specific for mouse Keratin 8, Rabbit polyclonal antibody against mouse keratin 5 and antifade reagent with DAPI to stain DNA. Both primary antibodies work best at 1:500 dilution. A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.