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- Question 7 A series of mutant strains (a through f) of a particular mold are not able to grow on minimal media unless they are supplemented by one or more of the compounds Q through V as shown below. Strain a b с d e f Q 0 0 + 0 0 + R 0 + + 0 + + Supplements S 0 o 0 0 0 0 T + + + + + + U V 0 + + 0 0 + 0 + + + + + + = growth 0 = no growth Draw a plausible metabolic pathway supported by the data provided in the Table above, showing the position of each metabolic intermediate in the pathway, which step is blocked by each mutant and explain your answer. (8 marks)Question 12 (1 point) Listen You are working in the lab with Streptococcus pyogenes and two other bacteria. You streak all three of these microbes onto a Blood Agar plate and observe the following: All of the bacteria grow on the plate but there is a large zone of clearing around S. pyogenes, and no zone of clearing on the other two bacteria. Based on these results what type of Medium is Blood Agar? O A) Blood agar is a culture medium. B) Blood Agar is a selective medium. C) Blood agar is a differential medium. O D) Blood agar is an exclusion medium. O E) Blood agar is a counting medium. 98% 99+QUESTION 9 Based on the results of the Kirby-Bauer test shown below, what can we say about this strain of bacteria? Mark all that are true. G. O Antibiotic C would be a good anitbiotic to use if you wanted to kill this bacteria. O This strain is resistant to antibiotic D B. D. E. F.
- Question 5 You spread a small amount of a water sample from the environment on an enriched media agar plate. After 24 hours, the plate is covered with many small yellow colonies. Does this plate contain a pure culture of bacteria? ○ No, because it came from the environment, there are lots of different organisms. ○ We know there are multiple species present, but each colony is a different species. ○ Yes, we know the plate contains a pure culture because there are individual colonies. ○ We do not know if the plate contains a pure culture or a mixed culture. or even if individual colonies are pure or mixed based on their appearance alone.Question 6 In the first part of the experiment, there were only a few colonies on the streptomycin positive plate. In the second part, there are a large number of colonies on the streptomycin positive plate that was inoculated with the antibiotic resistant strain. What is the most likely explanation for this difference? 1. The antibiotic resistant strain was composed of a large number of individuals with resistance to streptomycin 2. The exposure to streptomycin caused a mutation in a few individualsQuestion 7: A. Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder). B. Calculate how to make a series of 1:10 dilutions of this stock. Imagine you'll use 5 ml of each dilution for your experiment. Calculate the final concentrations of each tube. tube 1: take some stock, dilute it 1:10 with some water in a new tube - #1 tube 2: take some of #1, dilute it 1:10 with some water in a new tube tube 3: take some of #2, dilute it 1:10 with some water in a new tube tube 4: take some of #3, dilute it 1:10 with some water in a new tube C. First, decide the total volume to make of each tube-keep in mind measurement accuracy (consider preparing 20% more solution than what you will use). D. Then, calculate how much stock, water and final concentration of each. E. Then, instead of doing a serial dilution, calculate making each of the above solutions using the weight/volume % solution method. F. Compare and contrast serial dilution and W/V % methods
- Question 23 to do the catalase test, you should add______ (reagent) to your bacteria. If you see bubbles, this tells you for each choice, the item/phrase before the semicolon goes in the first space; the item/phrase after the semicolon goes in the second space. . O iron chloride/FeC13 (iron chloride); bacteria has NO catalase Kovac's reagent; bacteria has tryptophanase Zinc; bacteria turned nitrogen into nitrogen gas hydrogen peroxide; bacteria has catalaseQuestion 5 Match the description to the correct medium This medium is enrichment for fastidious [ Choose ] bacterial pathogens and differential based on hemolysis [Choose ] Sabouraud Dextrose Agar, "Sab" agar This medium is selective based on salt/sodium chloride tolerance at 7.5 % Mannitol Salt Agar, "MSA" weight/volume and differential based on mannitol fermentation MacConkey Agar, "Mac" agar Blood Agar, "BA" This medium is selective based on the ability EMB Eosin Methylene Blue Agar to grow in bile salts and crystal violet and is differetial based on lactose fermentation Tryptic Soy Agar, TSA this medium is all purpose and can grow a wide range of bacteria and fungi [ Choose ] Question 6 Below is an image of 3 different bacteria, letter labeld, growing on MacConkey Note that hrQuestion 5 For a successful direct staining, one should A. use a minimal amount of water to speed up the air-drying step B. make a very thick smear of cells on a slide to facilitate finding the cells under a microscope C. heat-fix a smear for an extended time to ensure that the cells will be stuck firmly to the slide D. All of the above E. A and C
- Question 1 We have received a specialized blood sera that contains antibodies. We want to test whether the antibody will bind to a target on our cell culture. The company that harvested the material has reported that the harvest works best at a 1:250 dilution. What volume of blood sera should we add to make a total volume of 1ml of antibody containing culture media? You may give your answer in any unit you like, but remember to include the unit. Edit Format Table 12pt v Paragraph vQuestion 1 A. Please indicate what the acronym, “VNTR” represents. (e.g., what words does this abbreviation represent.), then DEFINE “VNTR” and EXPLAIN the reason that VNTRs are useful in DNA fingerprinting. B. Is the male Rottweiler in the possession of Sam Slimy actually Goliath? This is the dog labeled “male Rottweiler” on the gel. Please offer evidence in support of your answer. C. What conclusions can you draw concerning the paternity of puppy #1? Please explain.Question 33 Orciprenaline (C₁₁H₁7NO3, 211.258 g/mol) is a bronchodilator used in the treatment of asthma. The BP assay for orciprenaline tablets is described below. HO The following information was obtained during the assay: Weight of 20 tablets = 2.5564 g Weight of tablet powder assayed = 0.5266 g Absorbance reading - 0.5868 Stated content per tablet = 20 mg A (1%, 1 cm) at 276 nm = 72.3 OH H N OH "Weigh and powder 20 tablets. Shake a quantity of the powder containing 80 mg of orciprenaline sulphate with 50 mL of 0.01 M hydrochloric acid (HCI), filter and add sufficient 0.01 M HCI to the filtrate to produce 100 mL. Dilute 10 mL to 100 mL with 0.01 M HCI and measure the absorbance of the resulting solution at the maximum at 276 nm." Orciprenaline 4 Answer the following questions, explaining your reasoning clearly. a) Calculate the amount of orciprenaline in the tablet powder analyzed. b) Calculate the average amount of orciprenaline per tablet. c) Calculate the percentage of the stated…