Rf Sample values A 0.21 В 0.39 C 0.33 0.27 Four amino acid samples were subjected to a normal-phase chromatography set up yielded the following Rf values. Which among the samples is the least polar? O and O A B. OD
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- Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…Figure 3: ● ● ● ● ● ● KDa ● 97.4 66.2 45.0 ● 31.0- 21.5 14.4 S-1 p-1 S-2 2-0 This figure was generated by centrifuging a pura sample of protein, removing the supernatant, and resuspending the pellet in the same volume as the supernatant to allow direct comparison. The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The text or number after the dash represents a different condition. For example, s-1 and p-1 are the supernatant and pellet samples under condition 1. It is not shown, but under wild-type conditions, essentially all of the protein is found in the supernatant. S-3 ● What does the intensity of each band represent? ● Would you find soluble protein in the supernatant or pellet? Why? Would you find aggregated protein in the supernatant or pellet? Why? For each condition (there are 5 different conditions), is there a higher percentage of the total protein…A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- 9026238page=9 La-donya 021 Exam Containers Undergraduate Exam Only Containers | S1, 2020/21 EXAM CHEM0901 | S1 2020/21 mistry) ISTRY A If the enthalpy of standard formation enthalpies of CO and CO, are -110 kJ mol and -393.5 kJ mol, respectively, what is the enthalpy change for the reaction? co+1/20 CO. (e) Select one O a 283.5 kJ mo Ob-283.5 kJ mol O C -503.5 kJ mol Od 141 8 k mol 223 pm 10/12/2020 A ENG irsert prt sc delete home 6 8 Y enter H. K pause ctrl altAll nucleic acid samples regardless of conformation and type will use the same absorption constant of 50 ng/ul for A260-1. O True False11 of 11 What volume (in µL to 1 decimal place) of stock SybrSafe solution (3.4 mg/mL) would you add to 120 mL of agarose solution to give a final concentration of 0.4 pg/mL? Answer: Submit < Previous Question Assessment Navigator Questionmark Ondemand licensed to University of Dundee DII DD 80 000 000 F10 F8 F9 F7 esc F5 F6 F3 F4 F1 F2 24 ... 6 7 * 00
- Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.The order is for Moxam 5 gm IM every 8 hours. On hand is 10 gram of Moxam with the following directions. Add 8 mL of diluent to yield 10mL of reconstituted solution. How many mL will you give?80-000 A7000 MN KANAMYCIN INJECTION, USP *1 g/3 mL (333 mg/mL) For IM or IV Use 3 mL Vial Rx only 10 Vials Using the label above, how many mL of Kanamycin must be administered to a patient in which the orders read 300 mg IM stat. Give your answer to 2 decimal places. Type mL in the units box. Your Answer: Sterie "Each mL contains lenamycin sulate equiralent to 333 mg kanamycia. 0.45% sodium bisulfite added as an antioxidant, buffered with sodium citrate (2.2 %). Adjusted to pH 4.5 with H,S0, Kanamycin Injection should not be phys ically mixed with other antibaderial agents Usual Dosage: Intramuscubr 7.5 ng/ig of bady weight at 12-hour intervals. Do not axceed 1.5 grams daily. See insert for IV and other uses Vial stoppers do not contain natural nubber latex. Store at 20 to 25 C (68 to 77 F) [see USP Contrdled Room Temperature]. Avoid freezing. APP APP Pharmaceuticals, LLC Schaumburg, IL G0173 63323-359-03 42650D LOT/EXP
- The protein concentration of a known standard is 100mg/mL If you prepared a serial dilution, mixing 10μL of protein with 40μL of water what would be concentrations of the first 3 dilutions?Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____Cells in the human liver contain glycogen, a polysaccharide that is used as a source of energy. This is a model of a typical glycogen granule: 42 22 AWARD Edit View Insert Format Tools Table be *** www 27544 Hals De w Jay MASCH Abh P CON p bo H S ******** 124 3. P WH ** patink The G k page flees P ******* 44651 CAPE Porn Tele de to MATE www 74 H Supe This type of granule may contain up to 30,000 (3 x 104) glucose subunits, making up the glycogen chains. A. How many ATP's could theoretically be produced when a granule of glycogen is hydrolyzed to glucose subunits that then enter glycolysis and respiration? B. Glycogen metabolism is regulated by insulin and glucagon. Both of these hormones are produced by the pancreas and act as signals that interact with a transmembrane receptor on target cells. Intracellular events involve protein kinase A that activates or inactivates enzymes involved in glycogen synthesis or degradation. Briefly describe what protein kinase A does to these proteins.