Suppose that you have an enzyme concentration at the molarity calculated in question 2 andthe product that you monitor has a value of x = 6200 M1 cm1. You ran eight reactions and foundthe following:Reaction #[substrate] (μM)Slope (Abs/min)00.0002345678100.163200.288600.5861000.7402000.9205001.07810001.143M-4.45x10-MCalculate the values of Kcat and KM for the reaction (remember to include units). Show your plotand your calculations.

To calculate the values of Kcat and KM for the reaction, we can use the Michaelis-Menten…

Answered: Suppose that you have an enzyme…

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter12: Gravimetric Methods Of Analysis

Section: Chapter Questions

Problem 12.2QAP

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Describe the difference between graphs showing temperature versus reaction rate for an enzyme-catalyzed reaction and an uncatalyzed reaction.
Draw the nullclines and find equilibria of the following extensions of the basic disease model. Find the nullclines and equilibria of this model when α = 2.0, µ = 1.0, and k = 4.0.
Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the product that you monitor has a value of ε = 6200 M1 cm1. You ran eight reactions and found the following: Reaction # [substrate] (μM) Slope (Abs/min) 1 0 0.000 2 10 0.163 3 20 0.288 4 60 0.586 5 100 0.740 6 200 0.920 7 500 1.078 8 1000 1.143 M=4.45x10-M Calculate the values of Kcat and KM for the reaction (remember to include units). Show your plot and your calculations.
The enzyme urease increases the rate of urea hydrolysis at pH 8.0 and 20 °C by a factor of 1014. Suppose that a given quantity of urease can completely hydrolyze a given quantity of urea in 19 minutes at pH 8.0 and 20 °C. How long would it take for this amount of urea to be hydrolyzed in the absence of urease at the same temperature and pH in sterile conditions? Include two significant figures in your answer. timeuncatalyzed years * TOOLS x10
A certain drug binds to a protein with Kd = 850.0 nM. What initial concentration of the drug would you need to add to a 50.0 µM solution of the protein so that 95.0% of the protein molecules are drug-bound at equilibrium? A. 1.62 μM B. 16.2 μΜ C. 47.5 μΜ D. 52.6 μM E. 63.7 μΜ
The enzyme urease is widely used for determining urea concentration in blood. The Michaelis constant for urease at room temperature is 2.0 mM and k2 = 2.5 × 10^3 s^-1 at pH = 7.5. Based on the kinetic experiment, when 4.76 mM urease was used, the initial rate of the reaction was determined to be 8.1 M/sec. What is the concentration in unit of mM of urea in blood? Please keep your answer to two decimal place. Please convert all concentration unit to mM in calculation. The answer is 4.26 (I would need help getting this answer)
The speed with which the protein bovine serum albumin (BSA) moves through water under the influence of an electric field was monitored at several values of pH, and the data are listed below. What is the isoelectric point of the protein?pH 4.20 4.56 5.20 5.65 6.30 7.00Speed/(μm s-1) 0.50 0.18 -0.25 -0.65 -0.90 -1.25
7. A group of Colby students discovered a hydrolase that cleaves cellulose. After purifying the enzyme, an assay was performed by adding 0.1 μM of enzyme to the cellulose substrate concentrations shown below to characterize kinetic properties. [S] 2 x 10-6 M 4 x 10-6 1 x 10-5 2 x 10-5 4 x 10-5 1 x 10-4 2 x 10-3 1 x 10-² 4 x 10-² V (umol/min) 28 40 75 95 112 128 139 140 141 A. Use the data in the table above to estimate KM and Vmax for the enzyme. B. What is the keat of this enzyme? C. What is the catalytic efficiency of this enzyme?
7. Copper(I) chloride salt is added to a solution of pure water to make a saturated solution. Upon dissolving, the concentration of copper(I) ion was found to be 4.15 x 10-4. What is the Ksp of copper (I) chloride? ©2022 F'22/S'23 Chemistry II A: 1.72 x 10-7 131
The enzyme urease increases the rate of urea hydrolysis at pH 8.0pH 8.0 and 20 °C20 °C by a factor of 1014.1014. Suppose that a given quantity of urease can completely hydrolyze a given quantity of urea in 88 minutes at pH 8.0pH 8.0 and 20 °C.20 °C. How long would it take for this amount of urea to be hydrolyzed in the absence of urease at the same temperature and pH in sterile conditions? how many years?
7TA Which of the following is not true regarding enzymes? * They catalyze only a particular type of reaction They are destroyed after the completion of the reaction they catalyse They remain active even after separation from the source Their activity depends on the pH of the solution Which of these statements about enzyme- catalyzed reactions is true? * The activation energy for the catalyzed O reaction is the same as for the uncatalyzed reaction. The rate of a reaction decreases steadily with time as substrate is depleted. At saturating levels of substrate, the rate of an enzyme-catalyzed reaction is propeE
Use EXCEL to plot the data shown below and Determine Km for the enzyme-catalyzed reaction. [S] mM V mM/sec 0.86 1.2 2.42 0.2 0.33 2 4 2.69

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