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- Which Unknown (a, b, c, d, or e) matches the test results pictured here (same as previous question)? Sadan Unkdwin Carbohydrate Sugar total (g) Fiber Unknown (g) (g) Protein Fat 25 25 50 20 30 16 15 65 2 21 12 d. 75 75 26 e 15 70 1 Biuret Lodine BesedictsGroup of lipids identified in Acrolein TestIn a tabular form, enumerate other chemical qualitative tests for carbohydrates, discuss its principle, chemical reagents employed and its positive test result.
- BARFOED’S TEST FOR MONOSACCHARIDESPlace 1 mL (20 drops) of each 1% carbohydrates solution in separate test tubes. Prepare a control with 1 mL water. Add 4 mL Barfoed’s reagent. Place the test tubes in boiling water bath for 10 minutes. Remove the test tubes and cool in cold water. Record your observations. A precipitate of cuprous oxide which may be green to red, indicates that a monosaccharide is present. Record your result and conclusion. Identify the compounds that are monosaccharides. SELIWANOFF’S TEST FOR KETOHEXOSESPlace 5 drops of 2% carbohydrate solution in separate test tubes. Prepare a control with 5 drops water. Add 1 mL of Seliwanoff’s reagent to each test tubes and heat. Record your results. The rapid formation of a bright red indicates that a ketohexose is present. Identify the compounds that are ketohexoses. IODINE TESTS FOR POLYSACCHARIDESPlace 2 mL of each carbohydrate solution in separate test tubes. Prepare a control with 2 mL of water. Add 1 drop of iodine solution…C. Mucic Acid Test for Galactose and Lactose describe the appearance of a few typical crystals under miscroscopeAfter how minutes the color is obtained with each carbohydrate in Resorcinol test?
- Reagents: 8 ml conc. Sulfuric acid, 1.5 ml alpha naphthol, 1 ml iodine in KI, 4 ml conc. HNO3, 4 ml Seliwanoff’s reagent, 3 ml Bial’s-orcinol reagent, SAMPLE: 5% of lactose, sucrose, glucose, galactose, fructose, maltose, starch and xylose ( 10 ml each) Materials: 12 test tubes 20 ml, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, 3 droppers, 250 ml beaker Procedure: 1. Molisch Test – (use all 8 samples) Place 1 ml of each sample in different test tubes. Add 1 drops of alpha napthol reagent in each tube. Mix thoroughly. Incline the tube and CAREFULLY allow 1 ml of conc. H2SO4 to flow down the side of the tube. Note the color formed at the zone of contact between 2 liquids. 2. Iodine Test – ( use all 8 samples) place 1 ml of the sample in a test tube. Add a drop of iodine. Observed color produced. Heat (direct heating) and note change in color. Cool and record again the result.…Match the objective of the test. -Mucic acid Test -Seliwanoff's Test -Bial's Test -Nylander's Test -Barfoed's Test Detects optical activity of reducing sugar Confirms the presence of pentose Differentiates reducing monosaccharide from reducing disaccharide Detects presence of glucose in urine Confirms presence of polyhydroxyaldehyde Identifies the presence of C=O in ketohexose Detects presence of brain sugar General test for polymers of polyhydroxy aldehyde and ketoneshttps://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Molish test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, the video is very brief) (please take time to answer)
- Qualitative Analysis of Carbohydrates HELP Select the test: Place test tubes in waterbath Fehling's test A Fehling' solution Fehling's solution Reset Glucose Lactose Sucrose Starch Qualitative Analysis of Carbohydrates HELP Reducing sugars (glucose & lactose) reduce the deep blue solution of copper (II) in the test reagent to red precipitate of insoluble cuprous oxide. Select the test: Fehling's test ResetDESCRIBE THE RESULTS OF THE REDUCTION TEST FOR SUGARS USING THE FOLLOWING TEST BENEDICTS TEST SUGAR RESULT 1. Glucose 2. Sucrose 3. Maltose 4. Fructose 5. Lactose 6. GalactoseBased on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Barfoed’s, test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.