The amino acid cysteine typically gives two spots in TLC. Explain why this is so.

Q: XI. Compare and contrast the following protein characterization techniques in terms of the…

A: The solution of the question is given below:  “Since you have posted a question with multiple…

Q: does melting point determination remove impurites in crude protein?

A: Concept- Melting point is the temperature at which solid will convert into liquid. Because it is a…

Q: Draw a chromatogram showing the spots when a mixture of the following compounds is separated using…

A:

Q: 2. The a chain of haptoglobin exhibits genetic polymorphism since glu- tamic acid can be replaced by…

A: 2)First, we will see the difference between the SDS and Native polyacrylamide gel electrophoresis…

Q: How would you make 2.0 L of electrotransfer buffer for transferring the proteins from a gel onto…

A: The composition of electrotransfer buffer is given as 1) Concentration of Tris (this is a solid; MW…

Q: The relative peak areas and retention for a mixture of five fatty acids separated by GC is given in…

A: Which Fatty Acid has the highest percentage? = FA 1

Q: viii) You have the following stock solutions available: 2.5M Tris, 0.5M Glycine and 10% SDS, how…

A: To prepare 1 liter solution of 25mM tris, 190mM glycine and 0.1% SDS can be prepared by following.

Q: Which technique measures secondary structure? a. Size exclusion chromatography b. Circular Dichroism…

A: The secondary structure is formed by bending of the 1o structure. In this, an intermolecular force…

Q: In polarimetry when lactic acid measured was contaminated with glycerol, what happens to the…

A:

Q: What are the applications of hplc?

A: High Performance liquid chromatography (HPLC) also known as High pressure liquid chromatography, is…

Q: A TLC is developed using a mixture of acetone/methanol (both polar solvents) as mobile phase. The…

A:

Q: 2. In what order would the following globular proteins emerge/eluted on gel filtration of a mixture…

A: Gel filtration technique elute or separate on the basis of molecular mass. Higher molecular mass…

Q: A chromatogram showing the results for an amino acid test show a solvent line of 7.5cm. If the amino…

A:

Q: pectin gel formation.

A: Pectin formed by hydrogen bonding between the corboxylic group of acids and hydroxyl group OH of the…

Q: During the experiment, a student calculated the R¡value for glutamic acid of 0.29 and 0.45 for…

A: Chromatography is defined as the laboratory technique for the separation of components of a mixture…

Q: What would be a suitable blank for an assay where the samples contain protein in a solution with dye…

A: A question based on absorption that is to be accomplished.

Q: What further testing should be done to extract and compare the composition of food dyes used In two…

A: Food coloring or the additives refers to a dye or a pigment or any substance which  imparts color to…

Q: What is in a fractionating column?     Silica gel     Collected fractions     Glass or plastic…

A: Using fundamental of fraction distillation process and hardware.

Q: Will a polar solvent, such as water, cause a difference in how the pigments are separated? Explain…

A: Polar solvents are those in which the dipoles exist. This dipole forms when the compound contains,…

Q: In a chromatography experiment measuring the polarity of amino acid funtional groups, one amino acid…

A: one amino acid had an Rf value of 0.32 and another had an Rf of 0.68

Q: In a mixture of the five proteins listed below, which should elute second in size- exclusion (gel-…

A: Size Exclusion Chromatography : It is a chromatographic method in which the components of the…

Q: Explain why the LC-MS-MS method is superior to the peptide mass mapping method.

A: Peptide mass fingerprinting (PMF) is an analytical technique for protein identification in which the…

Q: You compare the proteins from blood samples from 2 siblings; one with blond hair and the other with…

A:

Q: Explain how we would run a paper chromatography on an amino acid sample (explain the procedure or…

A: Paper chromatography is the chromatography based on liquid-liquid partition chromatography. It…

Q: Which of the following can determine location of glysosidic linkages?   A. Fractional analysis…

A: Fractional analysis using HPLC in tandem with MS HPLC stands for high performance liquid…

Q: 1. You know that these types of compounds can be analyzed by either GC or HPLC but you are told by…

A: GC can only apply to  analyse volatile substance while HPLC can handle any soluble compound…

Q: What are the chief differences between the Ninhydrin and Bradford assays for amino acid…

A: Ninhydrin and Bradford assay both are performed to determine amino acids. Nin hydrin reacts with…

Q: Draw TGA curves and also mentioned the loss of products for polyvinyl alcohol?

A: Thermal degradation of polyvinyl alcohol is studies in both solid and moleten state.

Q: Amino acids and fatty acids do not readily elute from gas chromatography columns even at…

A: Gas chromatography is a technique used to identify the presence of a compound in a mixture of…

Q: While purifying a new protein using a dextran Sephadex, the consistency of the column material…

A: As per the bartleby expert guidelines, I am allowed to answer one question at a time .  Please…

Q: What is the name of this oligopeptide? Use the three letter and one letter nomenclature of the amino…

A: Two amino acids are linked to each other through a peptide bond [C(=O)-NH-]. Hence "n" number of…

Q: Many ink dyes can be separated by the technique of___________ chromatography

A: Thin Layer Chromatography is a method for separating non-volatile substances. The separation…

Q: Proteins A, B, and C have isoelctric points of 8.2, 6.7,  and 5.2 respectively.  Place proteins A,…

A: The heavy molecules travel shorter distance whereas light molecules travel larger distance under the…

Q: A certain lipid sample consists of a wax of unknown molecular weight and composition. It is known,…

A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: Use the following chromatogram of standard amino acids: 24 45 16 19 21 12 15 23 13 14 18 10 12 14 16…

A: To determine the retention time of peak 4 from the given chromatogram.

Q: Question attached

A: Paper chromatography is a qualitative method in order to detect the presence of colored components.…

Q: The amino acid leucine has an Rf value of 0.73 and arginine has an Rf value of 0.20.  Draw their…

A: Rf value is the ratio of distance travel by the solute to the distance travel by solvent from base…

Q: b=3.50 = 3.50 3.30 d=335 나50. 7 3 2 1. 17-706 ppm 00

A: Glycerol is hygroscopic in nature due to extensive hydrogen bonding

Q: Image attached

A: In TLC, there are 2 phases used, i.e., mobile phase and stationary phase. Thin layer chromatography…

Q: Which might you need to do a ninhydrin stain to visualize spots in TLC?

A: The solutions used in TLC to detect whether a reaction is taking place or not might not have colour…

Q: what is The method that would be BEST to use to monitor protein secondary structure during the…

A: The secondary structure of the protein is affected by the complex folding properties of the protein…

Q: Q2) In the thin layer chromatography experiment, calculate the relative mobility for the two dyes if…

A: The relative mobility is calculated as : Rf = distance travelled by dye/distance travelled by…

Q: If the chromatogram was developed from reverse phase conditions, which of the following amino acids…

A: The reversed-phase conditions in chromatography indicates the hybrophobic stationary phase and…

Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography (b)…

A: Question is based on chromatography techniques:

Q: What does high NaCl do to protein that is bound to a Sp column?

A: Column chromatography: Column chromatography is used to separate charge analytes. Column…

Q: A) What are the basic principles underlying TLC technique? B) Draw a TLC plate and explain how TLC…

A:

Q: Please propose a chromatography-based strategy to purify human insulin proteins that have been…

A:

Q: If two samples have identical Rf values, in a Paper Chromatography of Amino Acids  lab procedure,…

A: The different amino acids can be separated by using the technique of Thin Layer Chromatography.…

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.