The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain staining and capsule staining. What result would you expect to see? a. none of these answers b. gram positive, endospore negative, acid-fast negative, capsule positive c. gram negative, endospore positive, acid-fast positive, capsule negative d. gram positive, endospore positive, acid-fast negative, capsule positive e. gram negative, endospore positive, acid-fast negative, capsule negative

The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain staining and capsule staining. What result would you expect to see? a. none of these answers b. gram positive, endospore negative, acid-fast negative, capsule positive c. gram negative, endospore positive, acid-fast positive, capsule negative d. gram positive, endospore positive, acid-fast negative, capsule positive e. gram negative, endospore positive, acid-fast negative, capsule negative

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2CS

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a. After conducting the Gram staining procedures and you observed that all cells are stained with too much purple though the bacteria you fixed is Gram negative, what could be the mistake you have done? b. After conducting the Gram staining procedures and you observed that all cells are stained with too much pink though the bacteria you fixed is Gram positive, what could be the mistake you have done?
Give 3 disease (each) that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore stain d. Capsule stain e. Flagella Stain
Give at least one disease that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore stain d. Capsule stain e. Flagella Stain
Which one of the following statements about the Gram stain is false? A-In the Gram stain, a dye called crystal violet is used to stain the cells purple. B-Gram positive cells end up being stained purple. C-Gram negative cells have thinner peptidoglycan layers than Gram positive bacteria. D-In the Gram stain, alcohol is used to kill the bacteria E-Gram negative cells end up being stained pink or red.
a. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below. b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown results
Answer this question xi) What is the most important step in the Gram staining procedure? Why?
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In a Gram-stained smear, medium-sized rods with rounded, pink ends were found, arranged chaotically. 1. What are the tinctorial properties of the bacteria from which the smear is made? 2. What is the purpose of studying the tinctorial properties of microorganisms? 3. How to stain the slide by the Gram method?
observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the above
How does the streak plate technique help in isolating individual colonies of bacteria? Why streak multiple times on one plate? 3. While observing a plate inoculated with bacteria you observe a white-tan fuzzy growth. What can you interpret from this observation? Explain how this outcome could’ve occurred. 4. What are 3 possible errors that could take place as you culture your samples which could lead to contamination? State 3 and explain why. Microbiology class
In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purple