The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.

Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter5: Abbreviations And Systems Of Measurement
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The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
Fluorescence
intensity
ROI
A
3
Time
Pre-bleaching Bleaching
imaging
imaging
B
Time
Post-bleaching
imaging
C
Time
D
Time
Transcribed Image Text:Fluorescence intensity ROI A 3 Time Pre-bleaching Bleaching imaging imaging B Time Post-bleaching imaging C Time D Time
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