The first step in identifying an unknown bacterium is to __________. culture your unknown on selective media perform endospore staining on your culture prepare a pure working stock simple staining on your culture acid-fast staining on your culture
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culture your unknown on selective media |
perform endospore staining on your culture |
prepare a pure working stock |
simple staining on your culture |
acid-fast staining on your culture |
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- Is is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or falseThe first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainPicture in white is acid fast other picure is negative stain so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _____??? negative stain: _____????
- After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred? Decolorizer was used after safranin Safranin was not used Crystal violet was not used The specimen was not heat-fixedIf some cells in your Gram stain are red/pink rods and some are purple cocci, __________. your smear was not made from a pure culture your unknown bacterium is a pleomorphic species the bacteria ferment sucrose your unknown is a species of Escherichia you should inoculate an EMB agar plateIn the image attached is a unknown bacteria lab. The gram stain result and growth result is already given to us and is shown. What other tests (1-2 examples) could we use to follow up this test. Explain why we should use those tests with justification.
- Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase TestTable 1. Different Staining Techniques Gram Stain Acid Fast Endospore Capsule Flagella 1. Principle 2. Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) 3. Stain/s Used 4. Type of Stain 5. Mechanism of Staining stain used) (of each 6. Flow of Method
- The unknown observations are shown as a mixed stain, gram positive, and gram negative. The next images shows the chart of what we can choose from for the gram positive bacterial stain. We are allowed to choose 4 test or stains that we would like to use to preform to identify the unknown.I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the gram stain process.You have identified two colony types A and B on the streak plate. Now briefly describe (in 3-4 sentences) the process of how you identified the cellular morphology/arrangement of each isolate. Again, assume you have all the necessary equipment and materials at your disposal. Be concise and thorough, not verbose; e.g., refer to the Gram stain, but describing the details of each step is not necessary.