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The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50

The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50
Transcribed Image Text:The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50
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