Be sure to answer all parts.Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems.(K, = 5.01 x 109 and pk, 8.3)%3D%3D(a) Calculate the pH of the Tris buffer after mixing 10.5 mL of 0.20 M HCI solution with 25.0 mL of0.10 M Tris.7.06(b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00020mol of H* was produced. What is the pH of the buffer at the end of the reaction?(c) What would be the final pH if no buffer were present?

Molarity is a measure of the concentration of a solution, expressed as the number of moles of solute…

Answered: Tris [tris(hydroxymethyl)aminomethane]…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A 40 umol.l-1 solution of flavin mononucleotide (FMN) gave an absorbance of 0.8 at 470 nm using a 1 cm cuvette. What is the molar extinction coefficient of FMN in M-1 cm-1?
Does this calculation look correct? My goal isto have a target mass of 10 µg of my protein with a total volume of 30 µl. Protein was measured and found to have a concentration of at 308.35 µg/ml Recall, mass = concentration * volume 10 µg = 308.35 µg/ml * V V = 0.01 mg/ 0.30835 mg/mL = 0.032430679 mL = 32.43067942 µL Note: cannot measure this amount; two dilutions required. Dilute by 1/40: measure 2.5 µl of protein at 0.30835 µg/µl and add it to 97.5 diluent to prepare a solution of protein at 7.71 mg/ml. This diluted sample will be used further. Mass = concentration * volume 10 µg = 7.71 mg/ml * V V = 0.01 mg/ 7.71 mg/ml = 1.3 µL Therefore, will measure 1.3 µl of protein at 7.71 mg/ml and add it to 28.7 µl of diluent.
More ratios. Through the use of nuclear magnetic resonance spectroscopy, it is possible to determine the ratio between the protonated and deprotonated forms of buffers. (a) Suppose the ratio of [ A- ]A I to [HA] is determined to be 0.1 for a buffer with pKar6.0.pKa = 6.0. What is the pH? (b) For a different buffer, 91974 suppose the ratio of [ A- ]lA J to [HA] is determined to be 0.1 and the pHpH is 7.0. In this case, what is the pKapKa of the buffer? (c) For another buffer with pKa=7.5PKa = 7.5 at pH 8.0pH 8.0, what is the expected ratio of [ A- ][A ] to [HA]? do
80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
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Paraphrasing Tool . medicinal meet Mniversity of Nizwa pKa of flurazepam is 8.71. Ifthe pH of the stomach is 3.5 and that of intestine is 6.0 then oral flurazepam will be t of Flurazepam Select one: a. ionized in the stomach and neutral in the intestine O b. neutral in the stomach and ionized in the intestine O c. ionized in both the stomach and the intestine O d. neutral in both the stomach and the intestine Which of the following molecules is neutral?
Size Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein Concentration 15.2 6.6 2 3.75 4.7 (mg/mL) Final Sample Volume 60 30 20 4 3 (mL) Enzyme Specific Activity 0.43 1.7 7.25 18.3 12.5 (units/mgprotein) Based on your protein purification sample data, in which of the purification steps in your protocol did you effectively purify your enzyme? Select all that apply. a) anion exchange correct b) cation exchange correct c) size exclusion 1 correct d) size exclusion 2
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Be sure to answer all parts. Calculate the equilibrium constants for decomposition of the following hydrogen halides at 298 K. Enter your answers in scientific notation. (a) 2HF(g) H2(g) + F2(g) 0.5 x 10 -97 (b) 2HCl(g) H2(g) + Cl2(g) 3.8 LOL -34 (c) 2HBr(g) H2(g) + Br2(g) 4.9 LO -20 (d) 2HI(g) H2(g) + I2(g) 1.1 -3
1- Using the pH 12 of the buffer and the pKa = 12.32. What is the molar ratio of A- to HA. 2- What is the molar amount of A- and HA needed to prepare a 0.2 M buffer? 3- How many grams of A- and HA are needed to prepare 40 mal of the buffer? The molar mass of HA is 142g/mol The molar mass for A- is 380.1g/mol
. A protein gives, under conditions of buffer composition, pH, and tem- perature that are close to physiological conditions, a molecular weight by sedimentation equilibrium measurements of 140,000 g/mol. When the same protein is studied by SDS gel electrophoresis in the absence or presence of the reducing agent B-mercaptoethanol (BME), the pat- terns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present, the stoi- chiometry of subunits, and the kinds of bonding (covalent, noncova- lent) existing between subunits. / A B - BME + BME STD Serum albumin, 67,000 Ovalbumin, 43,000 Carbonic anhydrase, 30,000 Trypsin inhibitor, 20,000 10 2. 4. Co cm migrated
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