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- Which compound elutes first and last in reverse chromatographyIn paper chromatography, a rectangular piece of filter paper serves as the mobile phase stationary phase eluent solute sample spotsUsing reversed phase chromatography in order to separate chloroquine and hydroxychloroquine. Which will be the first one to elute out of the column? Or both will elute the same time?
- Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatographyIn reverse phase chromatography which analyte will elute first, most polar or least polar?Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography (RPLC). CH3 CH3 p-xylene napthalene CH3 anisole OH phenol anisole p-xylene Elutes first Elutes last Answer Bank naphthalene phenol
- What is the stationary phase in thin layer chromatography and its polarity? Choices: Cellulose - non-polar Cellulose - polar Silica - non-polar Silica - polar6. A certain mixture contains the following compounds: propionic acid, 2-hexanol, isoamyl acetate, and 3,4-dimethylheptane. Rank the compounds in the order of decreasing rate of elution using normal phase liquid chromatography. Explain your answer.In a reverse phase chromatography setup, which of the following compounds will have the HIGHEST retention time? ethanol water toluene isopropanol
- Which is the correct order of elution of a gas chromatography that has a polar column that contains water, acetone, and n-propanol.In a reverse phase chromatography setup, which of the following compounds will have the HIGHEST retention time? water toluene isopropanol ethanolWhich of the following chromatographic methods cannot use a mixture of liquid solvents (eluents) during the separation process? Thin layer chromatography High pressure liquid chromatography Column chromatography Gas chromatography