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Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions?
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- Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions? (please indicate the solution and formula)What are the formulation components of a small volume Parenterals (SVP) product? Give flow diagrams for manufacturing SVP solution in ampoules? Please answer at your own easy words.Nicotiana tabacum cells are cultured to produce a polysaccharide gum. The reactor used is a stirred-tank reactor with an initial volume of 100 L. The maximum specific rate of growth of the culture is 0.18 d-1 and the yield coefficient of substrate in biomass is 0.5 gX/gS. The concentration of the limiting substrate in the medium is 3% (m/v). The reactor is inoculated with 1.5 g/L of cells and operated in batch until the substrate is exhausted, when the medium is fed with a constant flow rate of 8 L/d. The fed batch occurs in a quasi-steady state condition.a) Estimate the time of the batch step and the concentration of cells reached in this phase, considering exponential cell growth.b) The fed batch phase is carried out for a period of 40 days. What is the final concentration of cells in the reactor?c) The bioreactor is available for the process for 275 days a year, with an interval of 24 hours between each cultivation. What is the most advantageous operating mode for the process…
- Differentiate Wintrobe from Westergreen regarding the accuracy of the test in a tabulated order. Why do men have lower ESR values than women?You resuspend 1 ml of of a purified chlorophyll sample in 10 ml of 80% acetone, and determine that the optical density of the solution (at 652 nm) is 0.88. What is the concentration of your original preparation?What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its basic principles and general methods involved.
- In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the Van-Deemter equation.Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206
- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into account the dilution factor (100), and the total volume of extract (140).Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non- fastidious microorganisms (Merck, 2000). Its ingredients are listed in Table 2. You are tasked to prepare 300ml of NA, determine the amount of ingredients and write in column 3 in Table 2. Show your calculations. *A sample calculation was made for you. Peptone = 300 ml x 0.5% = 1.5 g Table 2. Medium Composition of Nutrient Agar (NA) with the recommended proportions (Merck, 2019) Ingredients In % In Grams/300ml Peptone 0.5 *1.5 Meat extract 0.3 Agar 1.5 Distilled Water As neededWhy are the chlorophylls less mobile on column chromatography, and why do they have lower Rf values than the carotenes?