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What are the differences between systematic and random errors and how do they effect accuracy and precision?
In what circumstances would you use standard addition (versus a normal calibration curve) to determine the amount of an analyte in a sample?
A urine sample, containing analyte Z is analysed by the standard addition method where 5 mL of the original sample was mixed with increasing amounts of a Z standard and each solution diluted to a volume of 50 mL prior to analysis. A plot of the final concentration of the standard in each of the 50 mL samples (x axis) versus
The measured signal from the analysis of each 50 mL sample (on y axis) produced a straight line with the general equation:
y = 44.72x + 4.06
what was the final concentration of Z in the 50 mL standard addition sample? what was the initial concentration of Z in the original urine sample?
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- You are preparing a 100.0 mL standard solution needed for titration analysis. The NaOH (40 g/mol) pellets are measured to be 0.5341 g in a balance with acceptable uncertainty of +0.001 g. Mass of the pellets is recorded after taring the container. The pellets are put in a beaker and added with 50 ml distilled water measured by a graduated cylinder. The solution is then quantitatively transferred to a 100-mL volumetric flask with uncertainty of ±0.08 mL and diluted to a 100-mL mark. What is the concentration (M) of the solution and estimate its uncertainty by propagation? Assumption: There is no uncertainty in molar mass. Express your answer as C+/- u M.An excess of 0.34 mg Pb is obtained from an assay test determined in an analysis. What is the percent relative error in ppt if the mass of the sample is 550 g?Norepinephrine can be analyzed in human urine. A fixed amount of DHBA was added to each sample and standard in a fixed concentration. Please explain the analytical function of adding DHBA. To the 1 mL of urine sample, small amounts of 1 µg/mL norepinephrine standard is added and the following results were obtained. What is the concentration of the norepinephrine in the urine sample with uncertainty?
- What are the advantages and disadvantages of volumetric and gravimetric analysis?2. The analytical ability of two lab technicians was compared by having each perform analyses for arsenic using the same method on samples having the same origin. For analyst A, s.d. = 0.12%, N=6; and for analyst B, s.d. = 0.06%, N-5. Do these results suggest a difference in the precision of the two chemists?You begin preparation of the calibration curve to measure absorbance vs concentration of FeSCN²+. To do so, you add 1.58 mL of 0.200 M Fe(NO3)3 to a cuvette and then directly add 316 µL of 0.001 M KSCN. What is the resulting concentration of FeSCN2+, assuming complete conversion of SCN´¯ to FeSCN²+´ Enter your answer in units of mM to four digits after the decimal.
- The method of standard additions was used to determine nitrite in a soil sample. A 1.00-mL portions of the soil extract sample was transferred to four 25-mL volumetric flasks. To each flask, increment volumes of a 1.00 x10-3M nitrite was added according to the table below. A colorimetric reagent was added to each flask to convert the nitrite into a colored product, and finally diluted to volume. What is the concentration of the nitrite in the soil extract based on the absorbance data below (with blank correction included)? Vol. of standard nitrite added, ml Absorbance 5.00 0.079 10.00 0.161 15.00 0.236 20.00 0.318 25.00 0.403Normality of a solution was determined by five different titrations. The results were 9.5 mL, 8.5 mL, 9.1 mL, 9.3 mL, and 9.1 mL. Calculate the mean, median and standard deviation of this dataThe protein concentration of a protein isolate was determined using the Bradford assay. Five dilution of bovine serum albumin (BSA) were prepared from a 5.00 mg/mL BSA stock solution. To these dilutions, 100 µl of Bradford reagent were added. After 5 minutes, the absorbance was taken at 595 nm. Standard # BSA, in mg/mL Volume of Bradford reagent, A595 in mL 1 5.0 0.000 1.00 5.0 0.134 3 2.00 5.0 0.265 4 3.00 5.0 0.388 4.00 5.0 0.497 The supernatant dilution was prepared by mixing 49 µL of the protein isolate with 16 µL water. The absorbance was taken 5 minutes after addition of 100 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original isolate if the absorbance at 595 nm was 0.413. Note: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of calculation.
- The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the instrument returned an error because the absorbance was too high. The sample was then diluted by using a pipette to take 100.0 μL�L of the sample and injecting it into a cuvette already containing 2.00 mLmL of water (total volume is 2.00 mLmL + 100.0 μL�L). The absorbance value of the diluted solution corresponded to a concentration of 6.22×10−6 M � . What was the concentration of the original solution?Analysis of a chromatogram of a mixture containing 0.0124 M analyte and 0.0448 M standard found that the peak area from the analyte was 324 and that the peak area from the standard was 258. In another experiment, a solution containing an unknown concentration of analyte and 0.0381 M standard was analyzed. The peak area of the analyte was 231, and the peak area of the standard was 197. What is the molar concentration of the unknown?Part C The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the instrument returned an error because the absorbance was too high. The sample was then diluted by using a pipette to take 100.0 µL of the sample and injecting it into a cuvette already containing 2.00 mL of water (total volume is 2.00 mL + 100.0 µL). The absorbance value of the diluted solution corresponded to a concentration of 8.57x10-6 M. What was the concentration of the original solution? Express the concentration to three significant figures with the appropriate units. • View Available Hint(s) HÀ ? original concentration of Fe2+ Value Units Submit