What is the method of MS protein quantification presented here

Anatomy & Physiology
1st Edition
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Chapter3: The Cellular Level Of Organization
Section: Chapter Questions
Problem 6RQ: Because they are embedded within the membrane, ion channels are examples of ________. receptor...
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What is the method of MS protein quantification presented here?

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Cell state 1
Cell state 2
Cell culture with
Cell culture plus,
for example,
'heavy' arginine
normal arginine
Combine cell lysates
and purify proteins
Protein digestion
LC-tandem-MS
of labelled peptides
"Light' peptide 'Heavy' peptide
Quantify levels
from peak ratio
m/z
Intensity
Transcribed Image Text:Cell state 1 Cell state 2 Cell culture with Cell culture plus, for example, 'heavy' arginine normal arginine Combine cell lysates and purify proteins Protein digestion LC-tandem-MS of labelled peptides "Light' peptide 'Heavy' peptide Quantify levels from peak ratio m/z Intensity
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ISBN:
9781938168130
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OpenStax College