What is the optimum pH to separate a mixture of lysine, arginine, and cysteine using electrophoresis? Draw the structures of the three amino acids in the protonation state that would predominate at the pH you have chosen.

What is the optimum pH to separate a mixture of lysine, arginine, and cysteine using electrophoresis? Draw the structures of the three amino acids in the protonation state that would predominate at the pH you have chosen.

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter22: Proteins

Section: Chapter Questions

Problem 22.68P

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Question

What is the optimum pH to separate a mixture of lysine, arginine, and cysteine using electrophoresis? Draw the
structures of the three amino acids in the protonation state that would predominate at the pH you have chosen.

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Step 1: Separation of amino acids by electrophoresis

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Step 2: Optimum pH for separating the three amino acids

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Step 3: Structure of the amino acids at pH 11

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