What would be the observed effects on the number of food vacuoles  of different concentrations such as 1% (v/v), 10% (v/v), and 25% (v/v) of India ink? Will it change over a duration of 30 minutes?

Nutritional Sciences: From Fundamentals to Food, Enhanced Edition
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ISBN:9781337486415
Author:McGuire
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Chapter2: Nutritional Assessment And Dietary Planning
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If there was India Ink added to a culture of Paramecium caudatum, it would increase the average number of food vacuoles because India ink contains no sugar or nutrients and the formation of food vacuoles would be the first stage in the metabolic process when digesting nutrient-rich particles. 

What would be the observed effects on the number of food vacuoles  of different concentrations such as 1% (v/v), 10% (v/v), and 25% (v/v) of India ink? Will it change over a duration of 30 minutes?

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