What would the order of elution be (first to last to come out of a size-exclusion chromatography column) for the mixture of myoglobin (17.7 kDa), hemoglobin (64.5 kDa), lysozyme (14.3 kDa) & albumin (67 kDa)? a. Albumin, hemoglobin, lysozyme, myoglobin Ob. Hemoglobin, myoglobin, lysozyme, albumin Oc. Lysozyme, myoglobin, albumin, hemoglobin Od. Albumin, hemoglobin, myoglobin, lysozyme
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- Relative to the position of hemoglobin A on your gel, where would you expect to find a mutant hemoglobin in which the b-chain glutamic acid residue was replaced by lysine? Sample Wall Number: 1. Normal Hemoglobin- Homoglobin AA 2. Sickle Trait Hemoglobin- Hemoglobin AS 3. Sickle Cell Hemoglobin- Hemoglobin SS 4. The unkniwn hemoglobin sample (showing the mutant sickle cell anemia trait)Carcinoembryonic antigen (CEA) is a protein biomarker related to colon cancer. Its concentration is known to be about 20 ng/mL in healthy people and higher than 2.5 µg/mL in the patients. The molecular weight of CEA is 180 kDa. What would be the molar concentrations (M or mole/liter) of 20 ng/mL and 2.5 ug/mL, respectively?1) What methods are used to measure glycosylated hemoglobin? 2) What potential conditions might cause erroneous results?
- How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4GenAlex Medical, a leading manufacturer of medical laboratory equipment, is designing a new automated system that can detect normal levels of dissolved immunoglobin A (70. to 312. mg/dL), using a blood sample that is as small as 250 µL. Calculate the minimum mass in milligrams of immunoglobin A that the new system must be able to detect. Be sure your answer has the correct number of significant digits. mg 0 x10 XAfter blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?
- I need to prepare a standard calibration curve for gamma globulin. absorbances on Y and mg of standard protein per assay on X. used 0.1mg/ml gamma stock for tubes 2-6. (Water (ml), gamma (ml), Abosrbance)--> (.036, .004, .290) (.036, .008, .358) (.024, .016, .341) (.016, .024, .520) (.008, .032, .597) - What is the math and how do you get the standard curve?a) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sampleThe proteins given below are separated using CM cellulose at pH 7.0 Write the order of elution of these proteins from the column Proteins pl Fibrinogen 5.8 Hemoglobin 7.1 Lysozyme 11.0 Pepsin 1.0 Ribonuclease 7.8
- Sketch the appearance after visualization of a protein mixture containing the seven proteins (fibrinogen, y-globulin, collagen, ovalbumin, myoglobin, hemoglobin, insulin) when subjected to two-dimensional (2D) gel electrophoresis.A hospital pharmacy has available 2-mL prefilled syringes containing 80 mg of tobramycin and 1.5-mL prefilled syringes containing 60 mg of tobramycin. The syringes are calibrated in 0.25-mL units. Explain how you would prepare a medication order calling for 110 mg of tobramycin to be added to 100 mL of D5W for intravenous infusion.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working