Which stains are differential in microbiology?
Q: How do you make liquid bacteria culture?
A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…
Q: What are the advantages of staining a bacterial preparation before observingit under a microscope?
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: Why can’t nitric acid stains be removed by washing?
A: Nitric acid: it has the chemical formula HNO3. It is a conjugate acid of nitrate and a protic…
Q: What is agar and why is it necessary for growing bacteria on plates?
A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: What substances control pigment production in a bacteria cell in microbiology?
A: An organism is a living thing that refers to prokaryotes and eukaryotes. A microscopic organism made…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: How does the UV radiation control the microbial growth affect of cells?
A: It is required to describe the affect of ultraviolet radiation controlling the microbial growth of…
Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…
A: The gram-positive and negative cells differ in their cell wall composition.
Q: Why are bacterial cells generally stained for microscopic viewing?
A: Staining bacterial cells prior to microscopic viewing is a routine process in most of microbiology…
Q: What is the color of endospores after Gram staining? After spore staining?
A: There are various staining techniques that are used to stain different cellular structures. The Gram…
Q: How do gram-positive and gram-negative bacteria differ in cellular structure, and how does this…
A: Gram staining is a most important technique in the microbiology to differentiate the group of…
Q: Under what circumstances staining is needed in bioprocesses?
A: A bioprocess is a process that utilizes complete living cells or their components (e.g., bacteria,…
Q: What are the applications for Pasteurization, Autoclaving, UV irradiation, and Gamma irradiation?
A: It is required to mention the application of Pasteurization, Autoclaving, UV irradiation, and Gamma…
Q: What is meant by Gram positive? Explain the mechanism of Gram positive staining.
A: Microorganisms or microbes are tangible objects that appear under a microscope. Large classes of…
Q: Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of…
A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: What are the purposes of carrying out the streak plate andpour plate techniques in microbiology?
A: It is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: How is the presence or absence of viable endospores related to sterility?
A: Endospore is a resistant asexual spore that develops inside the bacteria under the extreme…
Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Which stains are selective in microbiology?
A: INTRODUCTION Gram staining, acid fast staining, capsule staining and volutine granule staining are…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: what is Microscopy and Staining of Microbes
A: Microbes are the tiny unicellular ,prokaryotics organisms having diverse varieties and habitats.…
Q: What is Bacterial Injury in Microbiology?
A: Bacteria produced in pure culture under ideal circumstances are examined in the lab for their…
Q: What is the order of staining in microbiology
A: Crystal Violet - Primary stain ( Stain the cell wall of the microorganisms) Gram's iodine - Acts…
Q: what is sterilization or define sterilization?
A: Sterilization is defined as the process where all the living microorganisms, including bacterial…
Q: Why do we use oil with microscope ?
A: ••In light microscopy, in order to increase the resolving power we use oil Immersion techniques
Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…
A: The differential stain is used to distinguish between different structures and cellular components.…
Q: what color are endospores at the conclusion of the endospore stain?
A: Endospores are non-reproductive structures formed by the bacteria when they experience challenging…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: What types of compounds make up the gels used in electrophoresis?
A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…
Q: How is household antibacterial soap like a weapon against pathogenic bacteria?
A: Antibacterial soaps are those that contains certain chemicals that inhibit the growth of pathogens .
Q: What type of agar is MacConkey agar?
A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…
Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?
A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: what is the theory about the mechanism of the gram-stain reaction
A: Introduction :- A Gram stain test looks for bacteria in areas where an infection is thought to have…
Q: What products are sterilized using high energy ionizing radiation?
A: Sterilization is the process of killing microorganisms.
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: Discuss the importance of why flagellar stains must be freshly prepared?
A: Flagella Some bacteria, archaea, and eukaryotes whihcb are usually stayed to define as the microbe…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be found everywhere. They…
Q: What type of chromophore is associated with a negative stain?
A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…
Q: Why do acidic dyes not stain bacterial cells?
A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…
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