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Which the pigments present on spinach leaves correspond to each spot in the chromatogram resulting from TLC (based on the picture below)?
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- A student performed a chromatography experiment to determine the Rf value of an unknown amino acid. The distances traveled by the amino acid and the solvent line were 3.0 cm and 8.5 cm, respectively. What is the Rf value for this amino acid? 0.3 cm O 0.35 1.8 5.5 0.85 cm 0.65 O 2.81) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?use the following chromatogram and table as your data. What is the %composition of the Vitamin A peak in the sample? 78.43% 77.73% 7.867% 72.74%
- Research the pigments present on spinach leaves. Which among them correspond to each spot in the chromatogram resulting from TLC?4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.A student analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. OH Hollo HO OH OH OH NH₂ NH₂ NH₂ Threonine Valine Glutamic acid a. Identify spots A, B, and C in the developed TLC plate below based on the structures of the amino acids above (ie. which spot is threonine, valine, glutamic acid?). Hint: determine how the structures of the given amino acids differ A. BO С. b. Threonine can be phosphorylated using an enzyme called protein kinase. Given the same conditions the student used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Which of the following compounds would have the lowest Rf value in a TLC analysis? A. Hexane-2,3-diol B. Benzene C. Diethyl ether D. 1,3,5-cyclohexanetriol
- When spiking a sample with isopentyl alcohol, the isopentyl alcohol peak should increase in area on the chromatogram. O 1) True O 2) FalseA) What are the basic principles underlying TLC technique? B) Draw a TLC plate and explain how TLC can be used to identify the presence of a specific compound in an organic mixture.A semi-purified crude extract(SP) was tested against five known phenolic compounds, namely: (1) Quercetin, (2) Rutin, (3) Gallic acid, (4) Catechin, and (5) Ellagic acid. Answer the following questions: Based on the developed chromatogram, what can you infer about the semi-purified crude extract? Based on the developed chromatogram and your knowledge about TLC, what can you say about compounds 1 and 2? Is TLC the most accurate and reliable chromatographic technique used in analytical chemistry? If yes, why? If not, what is a more accurate and reliable chromatographic technique than TLC?
- Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC. -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.Give typed explanation 1. You must enter the range for a new lot of normal glucose control. The 20 results give you a mean of 80 mg/dL and a standard deviation of 4. What is your new range for normal glucose control?Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.