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Why is the plate count method a determination of the number of viable cells?
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- What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?What are the advantages and disadvantages of the viable plate count method?You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of media in the flask is 1L. You took an aliquot directly from the media and diluted it using trypan blue. Here are the numbers that you get: squares counted: 4 total cells counted: 988 total viable cells counted: 960 THESE ARE THE QUESTIONS: What is your cell viability? What is the total number of viable cells in your flask? Show all work. Given: Cell Viability = Viable Cells/Total Cells Cells/mL = Total Viable Cells/Coners Counted x 10,000 x Dilution factor
- When performing a Standard Plate Count, why are the counts reported as colony-forming units (CFUs) rather than cells?A.Why do you plate the cells from the viable count on LB agar without ampicillin? B.If you observe 100 colonies on your 1/100 plate, how many colonies do you expect if everything works perfectly on your 1/1000 plate?What does a white colony indicate during blue-white screening? Explain how the color is formed.
- You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IREWhy is that bacterial slide agglutination technique important in diagnostic procedure?With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?
- How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell at the same time? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/Please describe the pros and cons of the following methods for counting bacterial cells. 1) Petroff-Hausser counting chamber 2) Coulter Counter 3) Plate CountThere are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectively