You are given a microscope with a 150X NA 1.2 objective, a 400 nm laser and a slide containing very bright microparticles about 20 nm in diameter. You have one particle that emits at 450 nm and another particle at 500 nm. How close can the particles be and still be distinguished?

Physical Chemistry
2nd Edition
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Author:Ball, David W. (david Warren), BAER, Tomas
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Chapter11: Quantum Mechanics: Model Systems And The Hydrogen Atom
Section: Chapter Questions
Problem 11.61E: What is the physical explanation of the difference between a particle having the 3-D rotational...
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You are given a microscope with a 150X NA 1.2
objective, a 400 nm laser and a slide containing
very bright microparticles about 20 nm in
diameter. You have one particle that emits at 450
nm and another particle at 500 nm. How close
can the particles be and still be distinguished?
Transcribed Image Text:You are given a microscope with a 150X NA 1.2 objective, a 400 nm laser and a slide containing very bright microparticles about 20 nm in diameter. You have one particle that emits at 450 nm and another particle at 500 nm. How close can the particles be and still be distinguished?
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