You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
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You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
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- What are the importance of following the recommended sample preparation in performing serum protein electrophoresis? How does serum protein analysis helps the Patient with underlying conditions?FEHLINGS TEST You can use this as your reference : https://youtu.be/rKng5-ij6kQ1 ml of whole blood is collected and centrifuged to obtain a plasma sample. The volume of the plasma sample is 300 microlitres. A 1 in 4 dilution of the plasma is made and analysed using a cholesterol assay like the one you performed in the laboratory. 10 microlitres of both the diluted plasma sample and a cholesterol standard are assayed using 1mL of cholesterol reagent. Following incubation at 37 degrees Celcius, the samples are measured spectrophotometrically at 500nm. If the absorbance of the plasma sample assay was 0.5, the absorbance of the cholesterol standard assay was 0.4, and the concentration of cholesterol in the standard was 1.3mM, what was the concentration of the cholesterol in the whole blood (in mM)? (assume all of the cholesterol in the whole blood sample was recovered in the plasma; do not include units in your answer; give your answer to 2 decimal places)
- 2.4. Preparation of a Calibration CurveStandard Bovine Serum Albumin (BSA) solutionStock solution: 2.00 mg/mLStandard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSADiluent: distilled water a. on the picture b.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL ofdistilled water. Mix using a vortex mixer.c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of theremaining concentrations following the sample calculation. Table 2.4. Volumes needed for the preparation of the BSA standard solutions. Concentration to beprepared, mg/mL Volume of 2.00 mg/mLstock solution needed, μL Volume of distilledwater needed, μL 0.00 0 1000.00 0.125 62.5 937.5 0.25 0.50 1.00 1.50 2.00 please complete the given table with the correct computation like in the picture250- 1 2 3 4 5 6 -250 150- -150 100- -100 75- -75 50- 37- 25- 20- 15- 10 10- Std Biorad BSA Calf serum Fibrinogen Std Biorad Transferrin 161-0318 161-0374 -50 -37 -25 -20 15 10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data - measured distance moved and calculated Rr values Item Band Distance moved (mm) Rf Log 10 Mr (from graph) Calculated Mr (antilog of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 12. The following data were generated after a set of bovine serum albumin standards were measured via the BCA assay. Table 1. Absorbance measurements at 562 nm for BSA standards measured in triplicate in a BCA Assay. Standards A B C D E F G H I Conc (ug/ml) 2000 1500 1000 750 500 250 125 25 0 Replicate 1 Abs @ 562 nm 2.381 2.039 1.505 1.143 0.819 0.454 0.245 0.056 0 Replicate 2 Abs @ 562 nm 2.456 2.052 1.489 1.156 0.823 0.46 0.272 0.049 0 Replicate 3 Abs @ 562 nm 2.43 2.05 1.47 1.17 0.81 0.48 0.255 0.053 0.00 Use Excel or your graphing program of choice to create a standard curve using the data from table 1. Consider the best way to graph this data. Make sure the type of graph that you select will allow you to add a line of best fit. Display the equation for the line of best fit directly on the graph. Hint... you'll want to think about how to treat the triplicate values when making the graph. Also consider the type of graph that should be selected to allow for addition of the line of…