You obtain a calculated Vmax of 4.26uM/s and a Km of 122.5uM from a kinetics experiment performed using 0.5uM enzyme. What is the catalytic efficiency of this enzyme

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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You obtain a calculated Vmax of 4.26uM/s and a Km of 122.5uM from a kinetics experiment performed using 0.5uM enzyme. What is the catalytic efficiency of this enzyme? 

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Ans- In order t find out catalytic efficiency, first we have to calculate turnover number (Kcat) . 

Given: Vmax = 4.26uM/s 

           Enzyme Concentration (ET) = 0.5uM 

           Kcat = Vmax / ET  

                   = 4.26 uM/s / 0.5 uM 

                   = 8.52 sec-1 

 

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